Detailed information of TA system TA140347

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 448529..448754
• Replicon: chromosome
• Accession: NZ_CP044152
• Organism: Shigella flexneri strain AR-0425

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: F4V37_RS02440
• Protein ID: WP_000813254.1
• Coordinates: 448529..448684 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	448696..448754 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V37_RS02400	443655..444137	-	483	WP_000068107.1	ORF6N domain-containing protein	-
F4V37_RS02410	445426..445599	-	174	WP_000504450.1	hypothetical protein	-
F4V37_RS02415	445752..446306	-	555	WP_000640143.1	DUF1133 family protein	-
F4V37_RS02420	446303..446593	-	291	WP_000228038.1	DUF1364 domain-containing protein	-
F4V37_RS02425	446593..447192	-	600	WP_000940329.1	DUF1367 family protein	-
F4V37_RS02430	447326..448023	+	698	WP_223368647.1	IS1 family transposase	-
F4V37_RS02440	448529..448684	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
-	448696..448754	+	59	-	-	Antitoxin
F4V37_RS02445	449139..449504	-	366	WP_000610655.1	DUF551 domain-containing protein	-
F4V37_RS02450	449504..450169	-	666	WP_000208062.1	hypothetical protein	-
F4V37_RS02455	450166..450531	-	366	WP_001229297.1	HNH endonuclease signature motif containing protein	-
F4V37_RS02460	450533..450751	-	219	WP_000256998.1	DUF4014 family protein	-
F4V37_RS02465	450844..451200	-	357	WP_005048249.1	hypothetical protein	-
F4V37_RS02470	451258..451680	-	423	WP_001118168.1	DUF977 family protein	-
F4V37_RS02475	451695..452441	-	747	WP_000788996.1	ATP-binding protein	-
F4V37_RS25295	452587..452756	-	170	Protein_477	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	sitABCD	ipaH9.8	397305..456671	59366

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T140347 WP_000813254.1 NZ_CP044152:c448684-448529 [Shigella flexneri]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T140347 NZ_CP059125:296327-296500 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Nucleotide

• Length: 59 bp

>AT140347 NZ_CP044152:448696-448754 [Shigella flexneri]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATTTGTGAGACATAGATTGGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin