Detailed information of TA system TA140329

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1884572..1885299
• Replicon: chromosome
• Accession: NZ_CP044151
• Organism: Shigella sonnei strain AR-0426

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: F4V38_RS10080
• Protein ID: WP_000547563.1
• Coordinates: 1884988..1885299 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: F4V38_RS10075
• Protein ID: WP_000126296.1
• Coordinates: 1884572..1884991 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V38_RS10055 (1879624)	1879624..1880151	-	528	WP_001078777.1	electron transport protein HydN	-
F4V38_RS10060 (1880298)	1880298..1881308	-	1011	WP_001402444.1	DNA-binding transcriptional regulator AscG	-
F4V38_RS10065 (1881568)	1881568..1883025	+	1458	WP_001107890.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
F4V38_RS10070 (1883034)	1883034..1884458	+	1425	WP_119170825.1	6-phospho-beta-glucosidase AscB	-
F4V38_RS10075 (1884572)	1884572..1884991	-	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
F4V38_RS10080 (1884988)	1884988..1885299	-	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
F4V38_RS10085 (1885461)	1885461..1886234	-	774	WP_001026444.1	hypothetical protein	-
F4V38_RS10090 (1886259)	1886259..1886729	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
F4V38_RS10095 (1886722)	1886722..1887132	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
F4V38_RS10100 (1887129)	1887129..1887896	-	768	WP_000067403.1	formate hydrogenlyase subunit HycG	-
F4V38_RS10105 (1887896)	1887896..1888438	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
F4V38_RS10110 (1888448)	1888448..1890145	-	1698	WP_001288143.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T140329 WP_000547563.1 NZ_CP044151:c1885299-1884988 [Shigella sonnei]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T140329 NZ_CP059119:1860758-1861012 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT140329 WP_000126296.1 NZ_CP044151:c1884991-1884572 [Shigella sonnei]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT140329 NZ_CP059119:1860510-1860761 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22