Detailed information of TA system TA140314

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 63746..64172
• Replicon: plasmid pAR-0427-1
• Accession: NZ_CP044149
• Organism: Escherichia coli O157 strain AR-0427

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: F4V39_RS28665
• Protein ID: WP_001312861.1
• Coordinates: 63746..63904 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	63948..64172 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V39_RS28625	58790..59017	-	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
F4V39_RS28630	59111..59797	-	687	WP_000332487.1	PAS domain-containing protein	-
F4V39_RS28635	59991..60374	-	384	WP_001151564.1	relaxosome protein TraM	-
F4V39_RS28640	60660..61307	+	648	WP_072208664.1	transglycosylase SLT domain-containing protein	-
F4V39_RS28645	61603..62424	-	822	WP_021503380.1	DUF945 domain-containing protein	-
F4V39_RS28650	62535..62831	-	297	WP_001272251.1	hypothetical protein	-
F4V39_RS28660	63131..63508	+	378	Protein_80	hypothetical protein	-
F4V39_RS28665	63746..63904	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	63948..64172	-	225	NuclAT_0	-	Antitoxin
-	63948..64172	-	225	NuclAT_0	-	Antitoxin
-	63948..64172	-	225	NuclAT_0	-	Antitoxin
-	63948..64172	-	225	NuclAT_0	-	Antitoxin
F4V39_RS28675	64184..64903	-	720	WP_033807739.1	plasmid SOS inhibition protein A	-
F4V39_RS28680	64900..65334	-	435	WP_000845949.1	conjugation system SOS inhibitor PsiB	-
F4V39_RS28685	65389..67347	-	1959	WP_058653444.1	ParB/RepB/Spo0J family partition protein	-
F4V39_RS28690	67406..67639	-	234	WP_058653442.1	DUF905 domain-containing protein	-
F4V39_RS28695	67695..68222	-	528	WP_000290816.1	single-stranded DNA-binding protein	-
F4V39_RS29450	68567..68791	+	225	WP_001380475.1	hypothetical protein	-
F4V39_RS29455	68695..68979	-	285	WP_072165043.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA8 / blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR / tet(B)	-	1..74819	74819

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T140314 WP_001312861.1 NZ_CP044149:c63904-63746 [Escherichia coli O157]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T140314 NZ_CP059089:c2266594-2266412 [Pantoea agglomerans]
ATGAAGTACAGTGAATTCAGACGCTGGCTCATTCAGCAGGGCGCTATTTTGACTAAGCACCGCAGTGGAAGCAGCCATTT
CAAGGTCTTCCTGGGCGACAGGCAATCCATTTTTCCGGATCACGGAGCGAAAGAAATGCCTGAAGGTACGCGCAGAAAAA
TTTTAAAAGATCTGGGCCTGTGA

Antitoxin

Nucleotide

• Length: 225 bp

>AT140314 NZ_CP044149:c64172-63948 [Escherichia coli O157]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin