Detailed information of TA system TA140308

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 4928024..4928249
• Replicon: chromosome
• Accession: NZ_CP044148
• Organism: Escherichia coli O157 strain AR-0427

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: F4V39_RS24660
• Protein ID: WP_000813263.1
• Coordinates: 4928094..4928249 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	4928024..4928082 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V39_RS24620	4923299..4924390	+	1092	WP_001205823.1	hypothetical protein	-
F4V39_RS24625	4924397..4925143	+	747	WP_000788745.1	ATP-binding protein	-
F4V39_RS24630	4925165..4925935	+	771	WP_000450992.1	DUF1627 domain-containing protein	-
F4V39_RS24635	4925951..4926364	+	414	WP_001151233.1	DUF977 family protein	-
F4V39_RS24640	4926716..4927489	-	774	WP_000160654.1	alpha/beta hydrolase	-
-	4928024..4928082	-	59	-	-	Antitoxin
F4V39_RS24660	4928094..4928249	+	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
F4V39_RS24665	4928417..4928695	+	279	WP_001341388.1	hypothetical protein	-
F4V39_RS24670	4928697..4929746	+	1050	WP_001265172.1	DUF968 domain-containing protein	-
F4V39_RS24675	4929759..4930130	+	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
F4V39_RS24680	4930120..4930491	+	372	WP_000090264.1	antitermination protein	-
F4V39_RS24685	4930643..4931461	+	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
F4V39_RS24690	4931748..4931944	+	197	Protein_4708	TrmB family transcriptional regulator	-
F4V39_RS24695	4932082..4932795	+	714	WP_000261909.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T140308 WP_000813263.1 NZ_CP044148:4928094-4928249 [Escherichia coli O157]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T140308 NZ_CP059088:c24639-24319 [Pantoea agglomerans]
TTGTTCACCGTAAAGTTTCATGACGACGCTGGCGATGAGCTTGAAGTTTTGCCTGCTGATATTGCCGCGAAGATGGTCCG
TTTGCTTGAGAAACTTCAGGAAAATCCTCGTTCTCTGAGAGAGCCGCACTGTAAACCATTGGGCAATGGACTATTCGAGG
TTCGTACTATGGGCGGCGACATAGCACGCGGTTTGTGGGTCTATCAGACAGGTAAGAACATCTTCGTACTTCGTATTTTC
ATGAAAAAAACATCTAAAACGCCATTGGCAGAAATTAATTTGGCGTTTCGCAGACTGGAGGAGATGCTCGATGGCGCATA
A

Antitoxin

Nucleotide

• Length: 59 bp

>AT140308 NZ_CP044148:c4928082-4928024 [Escherichia coli O157]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin