Detailed information of TA system TA140304

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4034532..4035226
• Replicon: chromosome
• Accession: NZ_CP044148
• Organism: Escherichia coli O157 strain AR-0427

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: F4V39_RS20350
• Protein ID: WP_001263495.1
• Coordinates: 4034828..4035226 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: F4V39_RS20345
• Protein ID: WP_000554757.1
• Coordinates: 4034532..4034825 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V39_RS20325	4030243..4030740	+	498	Protein_3877	REP-associated tyrosine transposase RayT	-
F4V39_RS20330	4030885..4032597	-	1713	Protein_3878	flagellar biosynthesis protein FlhA	-
F4V39_RS20335	4032569..4033354	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
F4V39_RS20340	4033425..4034480	+	1056	WP_001226186.1	DNA polymerase IV	-
F4V39_RS20345	4034532..4034825	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
F4V39_RS20350	4034828..4035226	+	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
F4V39_RS20355	4035236..4035688	+	453	WP_001059871.1	GNAT family N-acetyltransferase	-
F4V39_RS20360	4036007..4036210	+	204	Protein_3884	RNA ligase RtcB family protein	-
F4V39_RS20365	4036209..4036730	+	522	Protein_3885	peptide chain release factor H	-
F4V39_RS20370	4036787..4038244	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
F4V39_RS20375	4038505..4038963	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	4039559..4039639	+	81	NuclAT_11	-	-
-	4039559..4039639	+	81	NuclAT_11	-	-
-	4039559..4039639	+	81	NuclAT_11	-	-
-	4039559..4039639	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T140304 WP_001263495.1 NZ_CP044148:4034828-4035226 [Escherichia coli O157]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T140304 NZ_CP059084:2307608-2307994 [Pantoea ananatis]
ATGGCAAAGCTCATTGCCTGGATAAACGGCGAGCGTACGGGAACGCTGACGCTGCATCACAACGGCGCGCTGCATTTCCA
GTATGACGCCAACTGGCTTCAGCATCCGCTTGCCAGACCTCTCTCGCTTTCGTTGCCCTTACAGCGCGCCGCGATGCATT
CTGAGAAAGTCGTGCACTTTTTCGAAAACCTTCTCTTTCCCAGTGCGTTGATCGAAAAGGATTTTTACCCCACTCACCCG
ATAAAATCCTGTGAGGTGTTTGCAGCATTGCAGAAAGTGGGACACACATTGCCCGGGGCGCTCAGTCTGCTGCCGGCAAA
GCGGCAGCCCGTCGCGCCGGACCAGGCAACGCACAGCGTCTTCAGCCCCGCCGATGAGGCACAGTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT140304 WP_000554757.1 NZ_CP044148:4034532-4034825 [Escherichia coli O157]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT140304 NZ_CP059084:2307369-2307608 [Pantoea ananatis]
ATGATTTACAGTCCCCGCCAGTTGGCGAATCAGGTCAGGCTATTGCGGCAGCAAAACGGCTGGACCCAGGTCGAACTGGC
GAAAAAAGTGGGCCTGAAACAGGCAACCCTGTCTCACTTCGAAAATGCCCCGGATACAACCTCACTGGCAACGTTGTTCA
AAATCTTACAGGCGCTCGATCTTGCTCTGTCGCTTCATGAAAACAGCGCATCCTCAGCCCCGGCTGAGGATGCCTGGTAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6