Detailed information of TA system TA140298

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-agrB/SymE(toxin)
• Location: 3680936..3681348
• Replicon: chromosome
• Accession: NZ_CP044148
• Organism: Escherichia coli O157 strain AR-0427

Toxin (Protein)

• Gene name: symE
• Uniprot ID: Q8FA88
• Locus tag: F4V39_RS18735
• Protein ID: WP_000132630.1
• Coordinates: 3680936..3681277 (-)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	agrB
Locus tag	-
Coordinates	3681272..3681348 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V39_RS18715	3676947..3678359	-	1413	WP_000199295.1	PLP-dependent aminotransferase family protein	-
F4V39_RS18720	3678536..3678700	+	165	WP_000394274.1	DUF1127 domain-containing protein	-
F4V39_RS18725	3678797..3679678	+	882	WP_001304007.1	DUF262 domain-containing protein	-
F4V39_RS18730	3679726..3680889	+	1164	WP_001304006.1	DUF1524 domain-containing protein	-
F4V39_RS18735	3680936..3681277	-	342	WP_000132630.1	endoribonuclease SymE	Toxin
-	3681272..3681348	+	77	NuclAT_14	-	Antitoxin
-	3681272..3681348	+	77	NuclAT_14	-	Antitoxin
-	3681272..3681348	+	77	NuclAT_14	-	Antitoxin
-	3681272..3681348	+	77	NuclAT_14	-	Antitoxin
-	3681272..3681348	+	77	NuclAT_15	-	Antitoxin
-	3681272..3681348	+	77	NuclAT_15	-	Antitoxin
-	3681272..3681348	+	77	NuclAT_15	-	Antitoxin
-	3681272..3681348	+	77	NuclAT_15	-	Antitoxin
F4V39_RS18740	3681498..3683252	-	1755	WP_000110082.1	restriction endonuclease subunit S	-
F4V39_RS18745	3683252..3684721	-	1470	WP_001302468.1	type I restriction-modification system subunit M	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002655 Escherichia coli O157:H7 str. EDL933 5456776..5475497 0 View NC_002695 Escherichia coli O157:H7 str. Sakai 5426911..5445631 0	inside	Genomic island	-	-	3670454..3689149	18695

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12294.13 Da
• Isoelectric Point: 8.4982

>T140298 WP_000132630.1 NZ_CP044148:c3681277-3680936 [Escherichia coli O157]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T140298 NZ_CP059083:c23509-23042 [Pantoea ananatis]
TTGAAGCTCTATCGCATGACCAAAACGCGCTATCTGGGGTCGGCCTGGAGCGGTTTTGGTGCCCGTGAAGGCGGCGGACG
CTGGAACAGCGTCGGCGTATCGATGGTCTACGCTTCTGAAACTGCTTCCCTCACCATGCTGGAGACGCTTATCCATCTGC
AAAGCGCCAGCGTGCTGGACTTTTTTACCCTCATGAGCATTGACGTTCCCGACAGGCTGATTGAATGGATTGATATCAAG
CAACTGCCTGACGACTGGGCCGCACCGGAAGCGCCTGCAGCGCTGCGTTTATTTGGTGATGCCTGGATACAAAGCGGGGG
ATCGGTCGCACTGCGCGTGCCCAGTGCCCTTTCGCCCGTGGAATCGAATTATCTTCTCAATCCCGAGCATCCTGAATTCA
GTGCGATCGTCAGACAGGCGGTAAACATCCCCTTCCTTTTTGACGCACGTTTTTCCCGACAGGTATAA

Antitoxin

Nucleotide

• Length: 77 bp

>AT140298 NZ_CP044148:3681272-3681348 [Escherichia coli O157]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A0E0Y5T0

Antitoxin