Detailed information of TA system TA140289

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 2273267..2274066
• Replicon: chromosome
• Accession: NZ_CP044148
• Organism: Escherichia coli O157 strain AR-0427

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: F4V39_RS11940
• Protein ID: WP_000347273.1
• Coordinates: 2273602..2274066 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: C3ST72
• Locus tag: F4V39_RS11935
• Protein ID: WP_001302819.1
• Coordinates: 2273267..2273602 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V39_RS11920	2269052..2269822	-	771	WP_001058231.1	2-dehydro-3-deoxyglucarate aldolase	-
F4V39_RS11925	2269838..2271172	-	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
F4V39_RS11930	2271547..2273118	+	1572	WP_001273776.1	galactarate dehydratase	-
F4V39_RS11935	2273267..2273602	+	336	WP_001302819.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
F4V39_RS11940	2273602..2274066	+	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
F4V39_RS11945	2274121..2274930	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
F4V39_RS11950	2275179..2276459	+	1281	WP_000681927.1	tagatose-bisphosphate aldolase subunit KbaZ	-
F4V39_RS11955	2276482..2276955	+	474	WP_001301475.1	PTS N-acetylgalactosamine transporter subunit IIB	-
F4V39_RS11960	2276966..2277745	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
F4V39_RS11965	2277735..2278613	+	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
F4V39_RS11970	2278631..2279065	+	435	WP_000948831.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T140289 WP_000347273.1 NZ_CP044148:2273602-2274066 [Escherichia coli O157]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T140289 NZ_CP059082:c1816742-1816488 [Halomonas titanicae]
ATGCGTGTTATATCGTTTACCGAAGCGCGTAATGGTCTAAAAGGCGTACTGGACGGTGTGGTCAATGATGCCGATACCAC
GATTATCACCCGCCGCGACGCAGAAGATGCAGTGGTCATGTCACTTGACTACTATAACAGCCTTATGGAAACCGTTTACT
TGATGCGCTCACCTGCTAACGCAGAGCACTTAAACAAGTCCATTGCTCAATACCGTGCAGGGCAAGCTCAACCAAGAGAG
CTAATGGATGAGTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12388.97 Da
• Isoelectric Point: 4.8616

>AT140289 WP_001302819.1 NZ_CP044148:2273267-2273602 [Escherichia coli O157]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT140289 NZ_CP059082:c1816495-1816232 [Halomonas titanicae]
ATGAGTAACCGCTTACTATGTTGGACTAACGAAGCTTGGAGCGACTACCTCTATTGGCAGGGGCAGGATAAAAAGACCTT
AAAGCGCATCAATAAACTTATCTTAGATACGCAGCGTTCTCCCTTTGAGGGCATTGGAAAACCTGAGCCCCTAAGGGAAA
ACCTCTCTGGCTTCTGGTCACGACGCATCGACGACTCGCATCGTCTGATTTATGCCGTCGATGAATCCACTCTCACTATC
ATTGCCTGTCGCTACCATTACTAA

Structures

Toxin

• AlphaFold DB: V0SSH7