Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2230502..2231229 Replicon chromosome
Accession NZ_CP044148
Organism Escherichia coli O157 strain AR-0427

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag F4V39_RS11720 Protein ID WP_000550189.1
Coordinates 2230915..2231229 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag F4V39_RS11715 Protein ID WP_000560249.1
Coordinates 2230502..2230918 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F4V39_RS11705 2225562..2227913 + 2352 WP_000695517.1 alpha-glucosidase -
F4V39_RS11710 2228439..2230457 + 2019 WP_000121479.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
F4V39_RS11715 2230502..2230918 - 417 WP_000560249.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
F4V39_RS11720 2230915..2231229 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
F4V39_RS11725 2231513..2232649 - 1137 WP_000018678.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
F4V39_RS11730 2232734..2233237 + 504 WP_001295542.1 M48 family metallopeptidase -
F4V39_RS11735 2233314..2234006 + 693 WP_000942544.1 vancomycin high temperature exclusion protein -
F4V39_RS11740 2234085..2235071 + 987 WP_000617675.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Antitoxin HigA

(1-138)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T140288 WP_000550189.1 NZ_CP044148:c2231229-2230915 [Escherichia coli O157]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T140288 NZ_CP059082:c1816495-1816232 [Halomonas titanicae]
ATGAGTAACCGCTTACTATGTTGGACTAACGAAGCTTGGAGCGACTACCTCTATTGGCAGGGGCAGGATAAAAAGACCTT
AAAGCGCATCAATAAACTTATCTTAGATACGCAGCGTTCTCCCTTTGAGGGCATTGGAAAACCTGAGCCCCTAAGGGAAA
ACCTCTCTGGCTTCTGGTCACGACGCATCGACGACTCGCATCGTCTGATTTATGCCGTCGATGAATCCACTCTCACTATC
ATTGCCTGTCGCTACCATTACTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15016.48 Da        Isoelectric Point: 4.5805

>AT140288 WP_000560249.1 NZ_CP044148:c2230918-2230502 [Escherichia coli O157]
MIAIADILHAGEKLTAVAPFLAGIQNEEQYIQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT140288 NZ_CP059082:c1816742-1816488 [Halomonas titanicae]
ATGCGTGTTATATCGTTTACCGAAGCGCGTAATGGTCTAAAAGGCGTACTGGACGGTGTGGTCAATGATGCCGATACCAC
GATTATCACCCGCCGCGACGCAGAAGATGCAGTGGTCATGTCACTTGACTACTATAACAGCCTTATGGAAACCGTTTACT
TGATGCGCTCACCTGCTAACGCAGAGCACTTAAACAAGTCCATTGCTCAATACCGTGCAGGGCAAGCTCAACCAAGAGAG
CTAATGGATGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References