Detailed information of TA system TA140275

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 301896..302121
• Replicon: chromosome
• Accession: NZ_CP044148
• Organism: Escherichia coli O157 strain AR-0427

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: F4V39_RS01430
• Protein ID: WP_000813258.1
• Coordinates: 301896..302051 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	302063..302121 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V39_RS01380	296900..297331	-	432	WP_001302123.1	tellurite resistance protein	-
F4V39_RS01395	297782..298495	-	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
F4V39_RS01400	298630..298827	-	198	WP_000917763.1	hypothetical protein	-
F4V39_RS01405	299052..299606	-	555	WP_000640035.1	DUF1133 family protein	-
F4V39_RS01410	299669..299974	-	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
F4V39_RS01415	299987..301036	-	1050	WP_001265229.1	DUF968 domain-containing protein	-
F4V39_RS01420	301038..301310	-	273	WP_000191871.1	hypothetical protein	-
F4V39_RS01425	301432..301776	-	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
F4V39_RS01430	301896..302051	-	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	302063..302121	+	59	-	-	Antitoxin
F4V39_RS01435	302342..302899	-	558	WP_000104474.1	DUF551 domain-containing protein	-
F4V39_RS01440	302901..303119	-	219	WP_000683609.1	DUF4014 family protein	-
F4V39_RS01445	303247..303558	-	312	WP_001289673.1	hypothetical protein	-
F4V39_RS01450	303551..303778	-	228	WP_000699809.1	hypothetical protein	-
F4V39_RS01455	303775..304056	-	282	WP_000603384.1	DNA-binding protein	-
F4V39_RS01460	304089..304805	-	717	WP_000450627.1	DUF1627 domain-containing protein	-
F4V39_RS01465	304839..305300	-	462	WP_000139447.1	replication protein	-
F4V39_RS01470	305293..306348	-	1056	WP_001356791.1	hypothetical protein	-
F4V39_RS01475	306417..306842	-	426	WP_000693878.1	Rha family transcriptional regulator	-
F4V39_RS01480	306826..307069	-	244	Protein_290	Cro/Cl family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	264451..369203	104752

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T140275 WP_000813258.1 NZ_CP044148:c302051-301896 [Escherichia coli O157]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T140275 NZ_CP059068:4487730-4487984 [Rhodoferax sp. AJA081-3]
ATGAAGGTCGTAACCTATTCCGAAGCCCGCAGCGCACTGAAAACAGTGCTGGATCGCGTGCATGACGATGCAGACGTGAC
GGTCATCAGCCGTCGCGATGGGGCTGATGCAGTCGTCATGTCGTTGGAGCACTACCAAAGCATCATGGAAACCATGCACC
TGCTTGGCACACCTGCAAATGCTGCGCATCTGGCCAAGTCCATTGCACAGCACAAAGCCGGCAAGGCCGTGCGGCGCAAG
TTGGTCCCAGCCTGA

Antitoxin

Nucleotide

• Length: 59 bp

>AT140275 NZ_CP044148:302063-302121 [Escherichia coli O157]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin