Detailed information of TA system TA140273

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 11170..11864
• Replicon: plasmid pAR-0428-2
• Accession: NZ_CP044147
• Organism: Escherichia coli O157 strain AR-0428

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: F4V40_RS29110
• Protein ID: WP_001263495.1
• Coordinates: 11466..11864 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: F4V40_RS29105
• Protein ID: WP_000554757.1
• Coordinates: 11170..11463 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V40_RS29085	6881..7378	+	498	Protein_8	REP-associated tyrosine transposase RayT	-
F4V40_RS29090	7523..9235	-	1713	Protein_9	flagellar biosynthesis protein FlhA	-
F4V40_RS29095	9207..9992	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
F4V40_RS29100	10063..11118	+	1056	WP_001226188.1	DNA polymerase IV	-
F4V40_RS29105	11170..11463	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
F4V40_RS29110	11466..11864	+	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
F4V40_RS29115	11874..12326	+	453	WP_001059871.1	GNAT family N-acetyltransferase	-
F4V40_RS29120	12645..12848	+	204	Protein_15	RNA ligase RtcB family protein	-
F4V40_RS29125	12847..13368	+	522	Protein_16	peptide chain release factor H	-
F4V40_RS29130	13425..14882	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
F4V40_RS29135	15143..15601	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	16197..16277	+	81	NuclAT_0	-	-
-	16197..16277	+	81	NuclAT_0	-	-
-	16197..16277	+	81	NuclAT_0	-	-
-	16197..16277	+	81	NuclAT_0	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	gmhA/lpcA	1..46899	46899

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T140273 WP_001263495.1 NZ_CP044147:11466-11864 [Escherichia coli O157]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T140273 NZ_CP059068:3874145-3874468 [Rhodoferax sp. AJA081-3]
ATGCAAACGCCTCCTACACTATCGCCAGCAAATGCTTGGCGACTGCAAGACGCCAAGGCGCAATTCAGCGAGTTGGTGGA
CAACGCCATGCGTGGCGTGCCCCAGCACGTCACGCGGCGTGGAAAACAAGCCGTGGTGGTGCTGTCGGAGGCAGATTTCG
AGGCACTGCAGAGAAATGCGGCCGCCAAGCAACCCAGGGGCCTGATCGAACACTTGCTGGCCATTCCAAAGACGCCCGTG
CCTTATCAGTCCAAAGAGCGCACGGTAGCGGACGCAGAGTTCCCCCGCCTTGACCTGCAGCCACGGGAGATTGACTTTTC
ATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT140273 WP_000554757.1 NZ_CP044147:11170-11463 [Escherichia coli O157]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT140273 NZ_CP059068:3874465-3874875 [Rhodoferax sp. AJA081-3]
ATGATCCTGCTCGATACCGTGGTTCTGTCGGAGTTGCGCAAGACAAAGCCGAATGCCAATGTGACAAACTATCTCAAAGC
GCAGGCCGAAGGCACCACATTCCTAAGTGCCTTGTCGATTGGTGAAATTGAAGCTGGCATTGAACGTCAGCGCGGTCACA
ATCCCGCGTTTGCGGACGAACTGGCGCAATGGTTGGTGGCATTGGAGCTGCAGTTCTCACAGTACATATTGCCGGTGACC
CCTGCGATCGCCAAACTATGGGGGCGGCTGTGTGTGCAGACCGGTAACAAGGGGATAGACAACCTGATCGCTGCAACCGC
GCTATGCCACAACCTCAGCGTTGTCACGCGCAATACCAAACACTTTGAACCTACGGGTGTTCGGGTGTTGAATCCGTTCG
ACGCGCAGTAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6