Detailed information of TA system TA140272

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 5207..5746
• Replicon: plasmid pAR-0428-2
• Accession: NZ_CP044147
• Organism: Escherichia coli O157 strain AR-0428

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: -
• Locus tag: F4V40_RS29070
• Protein ID: WP_001303998.1
• Coordinates: 5207..5467 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: C3TP07
• Locus tag: F4V40_RS29075
• Protein ID: WP_000729705.1
• Coordinates: 5486..5746 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V40_RS29055	2890..3468	+	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-
F4V40_RS29060	3573..4340	+	768	WP_001301698.1	class II glutamine amidotransferase	-
F4V40_RS29065	4311..5051	-	741	WP_001225679.1	murein L,D-transpeptidase	-
F4V40_RS29070	5207..5467	-	261	WP_001303998.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
F4V40_RS29075	5486..5746	-	261	WP_000729705.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
F4V40_RS29080	5932..6705	+	774	WP_000543891.1	C40 family peptidase	-
F4V40_RS29085	6881..7378	+	498	Protein_8	REP-associated tyrosine transposase RayT	-
F4V40_RS29090	7523..9235	-	1713	Protein_9	flagellar biosynthesis protein FlhA	-
F4V40_RS29095	9207..9992	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	gmhA/lpcA	1..46899	46899

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-85)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10026.71 Da
• Isoelectric Point: 10.3188

>T140272 WP_001303998.1 NZ_CP044147:c5467-5207 [Escherichia coli O157]
MNSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGT
HAALFG

Nucleotide

• Length: 261 bp

>T140272 NZ_CP059068:c2380554-2380054 [Rhodoferax sp. AJA081-3]
ATGACGTTCGGGGCTCCCGTTCCGTTACAGGCCTTGCACCGTCTCGACAGTTTCGATTGTGGAAAACCAAGCCTCAATGA
GTGGCTCACACGCTACGCACGCCAATCCCAGGCCAGCGGTTCTGCCAAGACCTTTGTGGTGGCGCACGATGACGGGGTGG
TTGGTTATTTCAGCCTGACGGTAGGCCAAGTCGATACCTTGGAGGCTCCCGAGCGCATCCGCAAAGGCATGGGCCAATAC
CCGGTTCCCGTTGTGATCTTGGCGCGCCTGGCTGTAGCACAACAGGCGCAAGGACATGGCGTAGGTATTGGCATGTTGCA
AGACGCGATTCGACGCACGCTGTTGATTGCCGAACAAGCTGGCATTCGCGCGCTGCTGACTCACCCGCTGGACGCGGACG
CGGCACGCTTTTACCTGCGGTTCGGTTTTGAGGCGTCACCATTGCGCCCGGATCAGCTTCTCTTGTTGCTGAAAGACGCC
CGCCGTTGGGTTCAGGTCTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9377.74 Da
• Isoelectric Point: 5.0758

>AT140272 WP_000729705.1 NZ_CP044147:c5746-5486 [Escherichia coli O157]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGV

Nucleotide

• Length: 261 bp

>AT140272 NZ_CP059068:c2380868-2380551 [Rhodoferax sp. AJA081-3]
ATGACTTCAGCAGTCGCCCCCCATACGCCTTCGGCACGTTCCGCCAATGCAGCGCGATCGGCCCGCTTGGGTTTGCGCGC
CACGGACCAGCAAGAGGCCATATTGCGCCGCGCCGCCGAGGTGGCCCACAAATCCCTGACAGACTTCATTCTGGACAGTG
CTTGTTTGGCGGCCGAACAAACCCTATTGGACCAGCGGTTGTTCATGGTGTCCGGTAGCCAATACCAGGCGCTAATGGAT
TTGCTGGATAGGCCCGCGCAGGACAACCCAGGCCTGAAAGACCTGTTTTCGCACAAGGCGCCCTGGGATAAACCATGA