Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4975035..4975532 Replicon chromosome
Accession NZ_CP044145
Organism Escherichia coli O157 strain AR-0428

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag F4V40_RS25850 Protein ID WP_001173343.1
Coordinates 4975359..4975532 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag F4V40_RS25845 Protein ID WP_000398619.1
Coordinates 4975035..4975331 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F4V40_RS25825 4971766..4972485 - 720 WP_000611288.1 two-component system response regulator DcuR -
F4V40_RS25830 4972482..4974113 - 1632 WP_001216477.1 sensor histidine kinase -
F4V40_RS25835 4974294..4974524 + 231 WP_000371704.1 (4Fe-4S)-binding protein -
F4V40_RS25840 4974536..4974808 + 273 WP_000405647.1 N-acetyltransferase -
F4V40_RS25845 4975035..4975331 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
F4V40_RS25850 4975359..4975532 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
F4V40_RS25855 4975651..4977168 - 1518 WP_001295074.1 lysine--tRNA ligase -
F4V40_RS25860 4977405..4978861 - 1457 Protein_4900 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4975035..4984384 9349


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T140266 WP_001173343.1 NZ_CP044145:4975359-4975532 [Escherichia coli O157]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T140266 NZ_CP059068:c819306-818821 [Rhodoferax sp. AJA081-3]
ATGAGTGAGCGTAAGAAGCCCAGCAGTCCGTCAGCGCGTTGGGTGGTGAATGGCTGGTCGGTATTTGCACATCCGCTGTT
TTTGGCCCAGTTGCAGACCCTAACCTTGCAGGTTGAAACTCTAAAGACCAAAGACCCGCAGGGCTACATCAAAAAGAACG
CGACCAAGCGTTTGGCGGCCATTCAAAAGTTGGCGTTTGAAGTCATTCCCCAAGACCCCACACGTGTCGAATACCGCCAG
GGCGGCACCTTGGGCGACGAATACAAACACTGGTTTCGCGCCAAGTTTTTTCAGCAATACCGTCTGTTCTTCCGATACCA
CCTGCAAGCAAAAATTATTGTGCTGGCGTGGGTCAATGACGACGACACTAAGCGTGCCTACGACAGCAGTGATGATGCCT
ACCGCGTATTCCGAAAAATGCTGGAGAGTGGGCATCCGCCGGATGACTGGGATGCGTTGTTGGCGCAAGCGAAGGCCAGC
AAGTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT140266 WP_000398619.1 NZ_CP044145:4975035-4975331 [Escherichia coli O157]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT140266 NZ_CP059068:c819628-819299 [Rhodoferax sp. AJA081-3]
ATGCCCACCACCATGGAAGTCGAATCGACCTTGACCGAACGCTACCAGACCACGGTGCCCGCCTCCGTACGCAGTGCGTT
GCGCCTGGGTAAACGCGACAAAATTCATTACAGCATCCGTCCTGGCGGCGAGGTGGTGTTGAGTCGAGCTGCCGATGTGG
AGGCGGACGACCCTGCGATGGGTGCATTTTTGGGCTTTCTGGCACACGACATGACCCAGCATCCGGAGCACCTGCAAGCG
GTGGATGCTGATTTGGTGGAGCGTATTCAGGCGCTGACCGCAGGTGTGGAGTTGGATATGGACGCGTCGTTGTCTCCTGA
CGATGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References