Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3780124..3780851 Replicon chromosome
Accession NZ_CP044145
Organism Escherichia coli O157 strain AR-0428

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag F4V40_RS20050 Protein ID WP_000550189.1
Coordinates 3780537..3780851 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag F4V40_RS20045 Protein ID WP_000560249.1
Coordinates 3780124..3780540 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F4V40_RS20035 3775184..3777535 + 2352 WP_000695517.1 alpha-glucosidase -
F4V40_RS20040 3778061..3780079 + 2019 WP_000121479.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
F4V40_RS20045 3780124..3780540 - 417 WP_000560249.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
F4V40_RS20050 3780537..3780851 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
F4V40_RS20055 3781135..3782271 - 1137 WP_000018678.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
F4V40_RS20060 3782356..3782859 + 504 WP_001295542.1 M48 family metallopeptidase -
F4V40_RS20065 3782936..3783628 + 693 WP_000942543.1 vancomycin high temperature exclusion protein -
F4V40_RS20070 3783707..3784693 + 987 WP_000617675.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Antitoxin HigA

(1-138)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T140258 WP_000550189.1 NZ_CP044145:c3780851-3780537 [Escherichia coli O157]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T140258 NZ_CP059060:919634-919912 [Synechococcus sp. LTW-R]
GTGATCCGCTCGTTTCGATGCGCAGACACCGAGCGACTAGCTCACGGATGGGCGATCCCCCGCTTTCAGGCCTTCGAACG
GGTGGCCCGGCGCAAGCTTCGCCAGCTGGACATTGCCTCGCGCCTGGAGGATCTGCGCATTCCCCCCGGCAATCGACTGG
AGGCACTGCGCGGCGACCGAGCTGGAGAACACAGCATCCGGATCAACGACCAATTCCGCCTTTGCTTTATTTGGTCAGAT
TCCGGCCCTGAAGCCGTTGAGATCGTTGATTACCACTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15016.48 Da        Isoelectric Point: 4.5805

>AT140258 WP_000560249.1 NZ_CP044145:c3780540-3780124 [Escherichia coli O157]
MIAIADILHAGEKLTAVAPFLAGIQNEEQYIQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT140258 NZ_CP059060:919912-920214 [Synechococcus sp. LTW-R]
ATGACTGCCACACATCGCCTAAGCCCTATTACCCCAGGTGAACTGCTCGTTGAGGAGTTTCTGGTTCCGCTTGGGGTCAG
CCAGTACCGACTGTCGAAGGCCATCAAGGTGCCGGCATCCAGGATCAGCGAAATCGTCTCCGGTCAGCGATCGATCACTG
CGGATACCGACCTGCGACTCTGCCGCTTCCTGGGCCTCAGTCCTGGGTACTGGCTTCGTGCTCAAGCCGCCTACGACACA
GAGATTGCTGGAGCGGAGCTCGCTGAGGAGATCGCCAAGATTCAGCCCTTGGTGAACAGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References