Detailed information of TA system TA140242

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1669623..1669994
• Replicon: chromosome
• Accession: NZ_CP044145
• Organism: Escherichia coli O157 strain AR-0428

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A0D6ZRD3
• Locus tag: F4V40_RS09020
• Protein ID: WP_001443846.1
• Coordinates: 1669845..1669994 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1669623..1669801 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V40_RS29395	1665375..1665545	+	171	WP_001625136.1	hypothetical protein	-
F4V40_RS08995	1665578..1666951	+	1374	WP_000123746.1	ATP-dependent RNA helicase DbpA	-
F4V40_RS09000	1667080..1668015	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
F4V40_RS09005	1668067..1669302	-	1236	WP_000040839.1	site-specific integrase	-
F4V40_RS09010	1669304..1669519	-	216	WP_000079604.1	excisionase XisR	-
F4V40_RS09015	1669619..1669807	-	189	WP_001302840.1	DUF1187 family protein	-
-	1669623..1669801	+	179	NuclAT_0	-	Antitoxin
-	1669623..1669801	+	179	NuclAT_0	-	Antitoxin
-	1669623..1669801	+	179	NuclAT_0	-	Antitoxin
-	1669623..1669801	+	179	NuclAT_0	-	Antitoxin
F4V40_RS09020	1669845..1669994	-	150	WP_001443846.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
F4V40_RS09025	1670050..1670859	-	810	WP_000166313.1	recombination protein RecT	-
F4V40_RS09030	1670852..1673452	-	2601	WP_000105140.1	exodeoxyribonuclease VIII	-
F4V40_RS09035	1673554..1673829	-	276	WP_001344816.1	hypothetical protein	-
F4V40_RS09040	1673904..1674074	-	171	WP_001352098.1	conserved protein, Rac prophage	-
F4V40_RS09045	1674074..1674295	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleA/espI / nleH2 / nleF	1655001..1779326	124325

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-48)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5383.12 Da
• Isoelectric Point: 8.3398

>T140242 WP_001443846.1 NZ_CP044145:c1669994-1669845 [Escherichia coli O157]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER

Nucleotide

• Length: 150 bp

>T140242 NZ_CP059045:c4024331-4023963 [Cronobacter sakazakii]
ATGACGATTCATTTTATCTCAGCGGAAGAGATAGTCCGTTTCCATGACCGTCTGTTGCAGGTCACGCCGGGCATTACCGG
TATGCCTGATCCAGGGCGGGCCGAAGCGCTGATGTACCGCGTGCTCAATAAATATGAGTATGAGGGCGTTAACGATATCT
GGCTGCTGGCGGCCATGCATTTGCTGGCGATTGCGCGCGGCCATATTTTTAACGACGGTAACAAACGTACGGCGCTATTC
ATTACACTGTTGTTTCTTAAGCGAAACGGTATTGATGTGGCGGCAAATCCGGCTTTTGTTGAGATGACCGTCAACGCGGC
GGCAGGGCGTTTGACGCTGGAAGAGATTGCTGCCCAGCTTCGCGCTTAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT140242 NZ_CP044145:1669623-1669801 [Escherichia coli O157]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A0D6ZRD3

Antitoxin