Detailed information of TA system TA140211

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1509701..1510395
• Replicon: chromosome
• Accession: NZ_CP044143
• Organism: Escherichia coli O157 strain AR-0429

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: F4V41_RS08895
• Protein ID: WP_001263495.1
• Coordinates: 1509701..1510099 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: F4V41_RS08900
• Protein ID: WP_000554757.1
• Coordinates: 1510102..1510395 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	1505288..1505368	-	81	NuclAT_12	-	-
-	1505288..1505368	-	81	NuclAT_12	-	-
-	1505288..1505368	-	81	NuclAT_12	-	-
-	1505288..1505368	-	81	NuclAT_12	-	-
F4V41_RS08870	1505964..1506422	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
F4V41_RS08875	1506683..1508140	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
F4V41_RS08880	1508197..1508718	-	522	Protein_1475	peptide chain release factor H	-
F4V41_RS08885	1508717..1508920	-	204	Protein_1476	RNA ligase RtcB family protein	-
F4V41_RS08890	1509239..1509691	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
F4V41_RS08895	1509701..1510099	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
F4V41_RS08900	1510102..1510395	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
F4V41_RS08905	1510447..1511502	-	1056	WP_001226188.1	DNA polymerase IV	-
F4V41_RS08910	1511573..1512358	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
F4V41_RS08915	1512330..1514042	+	1713	Protein_1482	flagellar biosynthesis protein FlhA	-
F4V41_RS08920	1514187..1514684	-	498	Protein_1483	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T140211 WP_001263495.1 NZ_CP044143:c1510099-1509701 [Escherichia coli O157]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T140211 NZ_CP059043:c863476-863258 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT140211 WP_000554757.1 NZ_CP044143:c1510395-1510102 [Escherichia coli O157]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT140211 NZ_CP059043:c863876-863502 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: S1FJN6