Detailed information of TA system TA140204

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 104057..104714
• Replicon: plasmid pAR-0429-1
• Accession: NZ_CP044142
• Organism: Escherichia coli O157 strain AR-0429

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: F4V41_RS00645
• Protein ID: WP_000270043.1
• Coordinates: 104364..104714 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: F4V41_RS00640
• Protein ID: WP_000124640.1
• Coordinates: 104057..104359 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V41_RS00590	99682..100110	+	429	WP_000591074.1	hypothetical protein	-
F4V41_RS00595	100167..100526	+	360	WP_000422768.1	hypothetical protein	-
F4V41_RS00600	100526..100972	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
F4V41_RS00605	100969..101487	+	519	WP_000210756.1	nitrite reductase	-
F4V41_RS00610	101487..101717	+	231	WP_000972663.1	hypothetical protein	-
F4V41_RS00615	101704..102561	+	858	WP_001167032.1	hypothetical protein	-
F4V41_RS00625	102792..103319	+	528	WP_001236377.1	thermonuclease family protein	-
F4V41_RS00630	103377..103649	+	273	WP_001043047.1	HU family DNA-binding protein	-
F4V41_RS00635	103737..104030	+	294	WP_001239997.1	chromosome segregation protein ParM	-
F4V41_RS00640	104057..104359	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
F4V41_RS00645	104364..104714	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F4V41_RS00650	104877..105424	+	548	Protein_119	transcriptional regulator	-
F4V41_RS00660	105765..105959	+	195	WP_000343597.1	hypothetical protein	-
F4V41_RS00665	105970..106341	+	372	WP_000516916.1	hypothetical protein	-
F4V41_RS00670	106334..106804	+	471	WP_001281821.1	hypothetical protein	-
F4V41_RS00675	106819..107154	-	336	WP_000683476.1	hypothetical protein	-
F4V41_RS00680	107251..107739	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
F4V41_RS00685	107742..108239	+	498	WP_000062185.1	hypothetical protein	-
F4V41_RS00695	108454..109158	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
F4V41_RS00700	109223..109402	-	180	Protein_127	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aadA7 / qacE / sul1 / floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / blaCMY-2	-	1..152532	152532

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T140204 WP_000270043.1 NZ_CP044142:c104714-104364 [Escherichia coli O157]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T140204 NZ_CP059042:1606-1899 [Acinetobacter baumannii]
ATGACTTATAAGCTTTTACGTCACAAGGACTTTACAGCAGAATGGGAAAAACTCCCTGTTGCTATACGGGATCAATTTAA
GAAGAAACTAGCCAAAGTCATAGAGCAGCCACATATTCCAAAGAATATGCTGAGAGGCGATCTTGCAGGTTGCTATAAAA
TTAAATTATTAAAAGCTGGTGTCAGACTTGTCTATCAAGTTAAAGATGATCAGGTAGTTATCTTGCTCATCACAGTCGGG
AAACGAGCTGATAGCATAGTTTATGACGAAGCTAAAAAGCGCATTAAAGACTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT140204 WP_000124640.1 NZ_CP044142:c104359-104057 [Escherichia coli O157]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT140204 NZ_CP059042:1371-1616 [Acinetobacter baumannii]
ATGGCTACTGTTAACTTCAGGGTCGATGAAGCCCTTAAAGAAAAATCCTATTCCATCCTTAAAGAACAAGGTATTGCACC
TACAGATTTCTTTACTAGTATTCTTGAGTATGTTGCTACGACTGGGAAACTACCTGTCAAAAAAGCTTTGCTTTCAGAAG
AAGATGAAGAATTGTTAGCTCTTGTCCGTAAGCGCATTAATGACCCTAAAGAGATGTTTGAGGAAGTCACATTAGATGAC
TTATAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9