Detailed information of TA system TA140201

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 28343..28568
• Replicon: plasmid pAR-0430-4
• Accession: NZ_CP044141
• Organism: Escherichia coli O157 strain AR-0430

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: F4V42_RS29785
• Protein ID: WP_000935259.1
• Coordinates: 28356..28568 (+)
• Length: 71 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	28343..28401 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V42_RS29735	23450..23734	+	285	WP_001208773.1	excisionase family protein	-
F4V42_RS29740	23787..25097	+	1311	WP_000013654.1	site-specific integrase	-
F4V42_RS29750	25663..26292	+	630	WP_000203825.1	anti-repressor protein	-
F4V42_RS29755	26340..26561	+	222	WP_000763353.1	TraR/DksA family transcriptional regulator	-
F4V42_RS29760	26558..26842	+	285	WP_001024844.1	DUF4752 family protein	-
F4V42_RS30135	26829..27298	+	470	Protein_48	ead/Ea22-like family protein	-
F4V42_RS30140	27320..27727	+	408	WP_001120841.1	DUF551 domain-containing protein	-
F4V42_RS29770	27727..28122	+	396	WP_000426668.1	hypothetical protein	-
-	28343..28401	-	59	NuclAT_0	-	Antitoxin
F4V42_RS29785	28356..28568	+	213	WP_000935259.1	type I toxin-antitoxin system Hok family toxin	Toxin
F4V42_RS29790	28688..29032	+	345	WP_000756595.1	YgiW/YdeI family stress tolerance OB fold protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	stx2B / stx2A	1..50155	50155

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (25-66)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7887.47 Da
• Isoelectric Point: 9.2654

>T140201 WP_000935259.1 NZ_CP044141:28356-28568 [Escherichia coli O157]
MLNTCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 213 bp

>T140201 NZ_CP059040:454270-454659 [Acinetobacter baumannii]
TTGATCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AGTTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATCACTCACAGAACACCATT
TCTCGCGTCAAAATTTTAAAAATAATTGATTATCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT140201 NZ_CP044141:c28401-28343 [Escherichia coli O157]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Antitoxin