Detailed information of TA system TA140186

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 2652069..2652868
• Replicon: chromosome
• Accession: NZ_CP044140
• Organism: Escherichia coli O157 strain AR-0430

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: F4V42_RS14120
• Protein ID: WP_000347273.1
• Coordinates: 2652069..2652533 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: C3ST72
• Locus tag: F4V42_RS14125
• Protein ID: WP_001302819.1
• Coordinates: 2652533..2652868 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V42_RS14090	2647070..2647504	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
F4V42_RS14095	2647522..2648400	-	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
F4V42_RS14100	2648390..2649169	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
F4V42_RS14105	2649180..2649653	-	474	WP_001301475.1	PTS N-acetylgalactosamine transporter subunit IIB	-
F4V42_RS14110	2649676..2650956	-	1281	WP_000681927.1	tagatose-bisphosphate aldolase subunit KbaZ	-
F4V42_RS14115	2651205..2652014	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
F4V42_RS14120	2652069..2652533	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
F4V42_RS14125	2652533..2652868	-	336	WP_001302819.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
F4V42_RS14130	2653017..2654588	-	1572	WP_001273776.1	galactarate dehydratase	-
F4V42_RS14135	2654963..2656297	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
F4V42_RS14140	2656313..2657083	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T140186 WP_000347273.1 NZ_CP044140:c2652533-2652069 [Escherichia coli O157]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T140186 NZ_CP059003:c4521459-4521172 [Escherichia coli]
ATAAGGACATGCCATGAGAACCCTGGTACAGGCTTTACGGAAGAAACAAAAAATACATTGGATCAAATATTTAAATCTCC
TGTTCCTCAAGGGATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTT
CGCGTTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCT
GTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12388.97 Da
• Isoelectric Point: 4.8616

>AT140186 WP_001302819.1 NZ_CP044140:c2652868-2652533 [Escherichia coli O157]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT140186 NZ_CP059003:c4521175-4520816 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACATTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTAAATACTTA
TATTATTGAAACGCTAAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: V0SSH7