Detailed information of TA system TA140176

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 928810..929349
• Replicon: chromosome
• Accession: NZ_CP044140
• Organism: Escherichia coli O157 strain AR-0430

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: -
• Locus tag: F4V42_RS05965
• Protein ID: WP_001303998.1
• Coordinates: 929089..929349 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: C3TP07
• Locus tag: F4V42_RS05960
• Protein ID: WP_000729705.1
• Coordinates: 928810..929070 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V42_RS05940	924564..925349	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
F4V42_RS05945	925294..927033	+	1740	WP_001301901.1	flagellar biosynthesis protein FlhA	-
F4V42_RS05950	927178..927675	-	498	Protein_849	REP-associated tyrosine transposase RayT	-
F4V42_RS05955	927851..928624	-	774	WP_000543891.1	C40 family peptidase	-
F4V42_RS05960	928810..929070	+	261	WP_000729705.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
F4V42_RS05965	929089..929349	+	261	WP_001303998.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
F4V42_RS05970	929505..930245	+	741	WP_001225679.1	murein L,D-transpeptidase	-
F4V42_RS05975	930216..930983	-	768	WP_001301698.1	class II glutamine amidotransferase	-
F4V42_RS05980	931088..931666	-	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-85)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10026.71 Da
• Isoelectric Point: 10.3188

>T140176 WP_001303998.1 NZ_CP044140:929089-929349 [Escherichia coli O157]
MNSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGT
HAALFG

Nucleotide

• Length: 261 bp

>T140176 NZ_CP059003:1705347-1705598 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9377.74 Da
• Isoelectric Point: 5.0758

>AT140176 WP_000729705.1 NZ_CP044140:928810-929070 [Escherichia coli O157]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGV

Nucleotide

• Length: 261 bp

>AT140176 NZ_CP059003:1705595-1705849 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA