Detailed information of TA system TA140175

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 922692..923386
• Replicon: chromosome
• Accession: NZ_CP044140
• Organism: Escherichia coli O157 strain AR-0430

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: F4V42_RS05925
• Protein ID: WP_001263495.1
• Coordinates: 922692..923090 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: F4V42_RS05930
• Protein ID: WP_000554757.1
• Coordinates: 923093..923386 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	918279..918359	-	81	NuclAT_12	-	-
-	918279..918359	-	81	NuclAT_12	-	-
-	918279..918359	-	81	NuclAT_12	-	-
-	918279..918359	-	81	NuclAT_12	-	-
F4V42_RS05900	918955..919413	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
F4V42_RS05905	919674..921131	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
F4V42_RS05910	921188..921709	-	522	Protein_841	peptide chain release factor H	-
F4V42_RS05915	921708..921911	-	204	Protein_842	RNA ligase RtcB family protein	-
F4V42_RS05920	922230..922682	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
F4V42_RS05925	922692..923090	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
F4V42_RS05930	923093..923386	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
F4V42_RS05935	923438..924493	-	1056	WP_001226188.1	DNA polymerase IV	-
F4V42_RS05940	924564..925349	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
F4V42_RS05945	925294..927033	+	1740	WP_001301901.1	flagellar biosynthesis protein FlhA	-
F4V42_RS05950	927178..927675	-	498	Protein_849	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T140175 WP_001263495.1 NZ_CP044140:c923090-922692 [Escherichia coli O157]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T140175 NZ_CP059003:c1479592-1479206 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT140175 WP_000554757.1 NZ_CP044140:c923386-923093 [Escherichia coli O157]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT140175 NZ_CP059003:c1479216-1478887 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6