Detailed information of TA system TA140095

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 775425..775839
• Replicon: chromosome
• Accession: NZ_CP044106
• Organism: Staphylococcus aureus strain FDAARGOS_660

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: FOB68_RS04220
• Protein ID: WP_001065046.1
• Coordinates: 775425..775676 (+)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FOB68_RS14510
• Protein ID: WP_000028424.1
• Coordinates: 775666..775839 (+)
• Length: 58 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOB68_RS04180	771151..772071	+	921	WP_031764964.1	recombinase RecT	-
FOB68_RS04185	772152..772760	+	609	WP_078090917.1	MBL fold metallo-hydrolase	-
FOB68_RS04190	772761..773231	+	471	WP_000610649.1	single-stranded DNA-binding protein	-
FOB68_RS04195	773261..774145	+	885	WP_000148302.1	DnaD domain protein	-
FOB68_RS04200	774152..774370	+	219	WP_000338528.1	hypothetical protein	-
FOB68_RS04205	774379..774783	+	405	WP_000401963.1	RusA family crossover junction endodeoxyribonuclease	-
FOB68_RS04210	774796..775164	+	369	WP_000101275.1	hypothetical protein	-
FOB68_RS04215	775168..775410	+	243	WP_000131381.1	phi PVL orf 51-like protein	-
FOB68_RS04220	775425..775676	+	252	WP_001065046.1	DUF1024 family protein	Toxin
FOB68_RS14510	775666..775839	+	174	WP_000028424.1	hypothetical protein	Antitoxin
FOB68_RS14515	776123..776284	+	162	WP_000889682.1	hypothetical protein	-
FOB68_RS04230	776299..776832	+	534	WP_001061844.1	dUTP diphosphatase	-
FOB68_RS04235	776869..777075	+	207	WP_000195810.1	DUF1381 domain-containing protein	-
FOB68_RS04240	777072..777221	+	150	WP_000595267.1	hypothetical protein	-
FOB68_RS04245	777380..778029	+	650	Protein_749	hypothetical protein	-
FOB68_RS04250	778029..778229	+	201	WP_000265041.1	DUF1514 family protein	-
FOB68_RS04255	778257..778673	+	417	WP_000590126.1	hypothetical protein	-
FOB68_RS04260	778905..779204	+	300	WP_000988336.1	HNH endonuclease	-
FOB68_RS04265	779335..779679	+	345	WP_000402904.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	sak / chp / scn	748130..803138	55008

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1024 (1-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9377.26 Da
• Isoelectric Point: 3.7128

>T140095 WP_001065046.1 NZ_CP044106:775425-775676 [Staphylococcus aureus]
MNNREQIEQSIISASAYNGNDTEGLLKEVEDVYKKAQAFDEILEGLPNAMQDALKEDIGLDEAVGIMTGQVVYKYEEEQE
NEY

Nucleotide

• Length: 252 bp

>T140095 NZ_CP058940:2669592-2670002 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 58 a.a.
• Molecular weight: 6468.29 Da
• Isoelectric Point: 4.2484

>AT140095 WP_000028424.1 NZ_CP044106:775666-775839 [Staphylococcus aureus]
MSISVGDKVYNHETNESLEIVQLVGDIRDTHYKLSDDSVISIIDFITKPIYLIKGDE

Nucleotide

• Length: 174 bp

>AT140095 NZ_CP058940:2669345-2669611 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA