Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 591398..592061 Replicon chromosome
Accession NZ_CP044101
Organism Citrobacter werkmanii strain FDAARGOS_616

Toxin (Protein)


Gene name higB Uniprot ID A0A2Z3X092
Locus tag FOB24_RS03505 Protein ID WP_038636359.1
Coordinates 591398..591700 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2Z3X2H4
Locus tag FOB24_RS03510 Protein ID WP_038636361.1
Coordinates 591720..592061 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOB24_RS03485 586941..588464 - 1524 WP_038636350.1 sugar ABC transporter ATP-binding protein -
FOB24_RS03495 588579..590843 + 2265 WP_150344515.1 hybrid sensor histidine kinase/response regulator -
FOB24_RS03500 590913..591242 + 330 WP_069325126.1 hypothetical protein -
FOB24_RS03505 591398..591700 + 303 WP_038636359.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FOB24_RS03510 591720..592061 + 342 WP_038636361.1 HigA family addiction module antidote protein Antitoxin
FOB24_RS03515 592113..592871 - 759 WP_150344516.1 phosphonate metabolism protein PhnP -
FOB24_RS03520 592880..593314 - 435 WP_150344517.1 aminoalkylphosphonate N-acetyltransferase -
FOB24_RS03525 593301..593855 - 555 WP_137347414.1 ribose 1,5-bisphosphokinase -
FOB24_RS03530 593858..594994 - 1137 WP_038636374.1 alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase -
FOB24_RS03535 594991..595671 - 681 WP_150344518.1 phosphonate C-P lyase system protein PhnL -
FOB24_RS03540 595778..596536 - 759 WP_003826710.1 phosphonate C-P lyase system protein PhnK -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Cro/C1-type helix-turn-helix domain

(23-70)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11856.50 Da        Isoelectric Point: 9.9581

>T140071 WP_038636359.1 NZ_CP044101:591398-591700 [Citrobacter werkmanii]
MSKSLNIRNFRDTWLEDFFERATPHRKIPADIHTALARKLDIINAAVSHRDLRSPPGNRYEELTGKLQEYSSIRVNKQYR
LIFKWVNGKAEDVYLDPHIY

Download         Length: 303 bp

>T140071 NZ_CP058886:c3611975-3611577 [Shigella flexneri]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13063.93 Da        Isoelectric Point: 7.2038

>AT140071 WP_038636361.1 NZ_CP044101:591720-592061 [Citrobacter werkmanii]
MKQATRKPTTVGDVLQYEYLEPLDLKINDLAEILHVHRNTVSALVNNNRKLTTDMAFRLAKAFDTSVDFWRNLQSAVDLW
EVENDMRAQEELNRIMSVKDFVAQRNVATKKVA

Download         Length: 342 bp

>AT140071 NZ_CP058886:c3612271-3611978 [Shigella flexneri]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Z3X092

Antitoxin

Source ID Structure
AlphaFold DB A0A2Z3X2H4

References