Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4874000..4874562 Replicon chromosome
Accession NZ_CP044098
Organism Citrobacter portucalensis strain FDAARGOS_617

Toxin (Protein)


Gene name higB Uniprot ID A0A1C0NXV4
Locus tag FOB25_RS23325 Protein ID WP_003833016.1
Coordinates 4874284..4874562 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A6H3AUF8
Locus tag FOB25_RS23320 Protein ID WP_003833018.1
Coordinates 4874000..4874284 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOB25_RS23300 4869579..4871681 + 2103 Protein_4480 formate dehydrogenase-N subunit alpha -
FOB25_RS23305 4871695..4872579 + 885 WP_003833024.1 formate dehydrogenase N subunit beta -
FOB25_RS23310 4872572..4873228 + 657 WP_003020054.1 formate dehydrogenase-N subunit gamma -
FOB25_RS23315 4873358..4873960 + 603 WP_032943476.1 inorganic diphosphatase -
FOB25_RS23320 4874000..4874284 - 285 WP_003833018.1 HigA family addiction module antidote protein Antitoxin
FOB25_RS23325 4874284..4874562 - 279 WP_003833016.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FOB25_RS23330 4874783..4876498 - 1716 WP_032943475.1 ATP-binding cassette domain-containing protein -
FOB25_RS23335 4876808..4878505 - 1698 WP_003833011.1 malate dehydrogenase -
FOB25_RS23340 4878685..4878825 - 141 WP_003833009.1 stationary-phase-induced ribosome-associated protein -
FOB25_RS23345 4879053..4879484 + 432 WP_005126807.1 OsmC family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-83)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10667.30 Da        Isoelectric Point: 9.8965

>T140068 WP_003833016.1 NZ_CP044098:c4874562-4874284 [Citrobacter portucalensis]
MIMNFRHKGLRDLFLHGRTSGVMAKHVRRLRHRLAVIDAASKVTDINMPGYKLHPLVGERDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T140068 NZ_CP058886:2594124-2594231 [Shigella flexneri]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10371.10 Da        Isoelectric Point: 8.8094

>AT140068 WP_003833018.1 NZ_CP044098:c4874284-4874000 [Citrobacter portucalensis]
MKMANHPRPGDIIQEALEELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPEMWLNLQNTWSLAEAQ
KSVDISRLRKLAVQ

Download         Length: 285 bp

>AT140068 NZ_CP058886:c2594077-2594011 [Shigella flexneri]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1C0NXV4

Antitoxin

Source ID Structure
AlphaFold DB A0A6H3AUF8

References