Detailed information of TA system TA140005

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 154998..155655
• Replicon: plasmid unnamed1
• Accession: NZ_CP044075
• Organism: Providencia stuartii strain FDAARGOS_645

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: FOB53_RS00975
• Protein ID: WP_000270043.1
• Coordinates: 155305..155655 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FOB53_RS00970
• Protein ID: WP_000124640.1
• Coordinates: 154998..155300 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOB53_RS00920	150623..151051	+	429	WP_000591074.1	hypothetical protein	-
FOB53_RS00925	151108..151467	+	360	WP_000422768.1	hypothetical protein	-
FOB53_RS00930	151467..151913	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
FOB53_RS00935	151910..152428	+	519	WP_000210756.1	nitrite reductase	-
FOB53_RS00940	152428..152658	+	231	WP_000972663.1	hypothetical protein	-
FOB53_RS00945	152645..153502	+	858	WP_001167032.1	hypothetical protein	-
FOB53_RS00950	153570..153716	+	147	WP_014342196.1	hypothetical protein	-
FOB53_RS00955	153733..154260	+	528	WP_001236377.1	thermonuclease family protein	-
FOB53_RS00960	154318..154590	+	273	WP_001043047.1	HU family DNA-binding protein	-
FOB53_RS00965	154678..154971	+	294	WP_001239997.1	chromosome segregation protein ParM	-
FOB53_RS00970	154998..155300	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
FOB53_RS00975	155305..155655	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FOB53_RS00980	155818..156366	+	549	WP_001061574.1	transcriptional regulator	-
FOB53_RS00990	156707..156901	+	195	WP_000343597.1	hypothetical protein	-
FOB53_RS00995	156912..157283	+	372	WP_000516916.1	hypothetical protein	-
FOB53_RS01000	157276..157746	+	471	WP_001281821.1	hypothetical protein	-
FOB53_RS01005	157761..158096	-	336	WP_000683476.1	hypothetical protein	-
FOB53_RS01010	158193..158681	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
FOB53_RS01015	158684..159181	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	qnrB4 / floR / sul2 / sul1 / qacE / ant(3'')-Ia / blaOXA-10 / cmlA1 / aac(6')-IIc / ere(A)	-	1..186363	186363

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T140005 WP_000270043.1 NZ_CP044075:c155655-155305 [Providencia stuartii]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T140005 NZ_CP058874:c689938-689474 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGCGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT140005 WP_000124640.1 NZ_CP044075:c155300-154998 [Providencia stuartii]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT140005 NZ_CP058874:c690273-689938 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCTGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9