Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 4203284..4203870 Replicon chromosome
Accession NZ_CP044039
Organism Klebsiella pneumoniae strain FDAARGOS_630

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag FOB38_RS22570 Protein ID WP_002920800.1
Coordinates 4203502..4203870 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag FOB38_RS22565 Protein ID WP_004174006.1
Coordinates 4203284..4203505 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOB38_RS22545 4199441..4200367 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
FOB38_RS22550 4200364..4201641 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
FOB38_RS22555 4201638..4202405 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
FOB38_RS22560 4202407..4203120 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
FOB38_RS22565 4203284..4203505 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
FOB38_RS22570 4203502..4203870 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
FOB38_RS22575 4204143..4205459 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
FOB38_RS22580 4205566..4206453 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
FOB38_RS22585 4206450..4207295 + 846 WP_004145129.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
FOB38_RS22590 4207297..4208367 + 1071 WP_032433652.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 4200364..4209104 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T139926 WP_002920800.1 NZ_CP044039:4203502-4203870 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T139926 NZ_CP058853:c1774008-1773901 [Shigella flexneri]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGGACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT139926 WP_004174006.1 NZ_CP044039:4203284-4203505 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT139926 NZ_CP058853:1774056-1774122 [Shigella flexneri]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References