Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 5220429..5221026 Replicon chromosome
Accession NZ_CP044033
Organism Klebsiella pneumoniae strain FDAARGOS_631

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag FOB39_RS25985 Protein ID WP_004142563.1
Coordinates 5220709..5221026 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag FOB39_RS25980 Protein ID WP_004142561.1
Coordinates 5220429..5220716 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOB39_RS25950 5216509..5216757 + 249 WP_002893037.1 DUF1158 domain-containing protein -
FOB39_RS25955 5216775..5217116 - 342 WP_004848570.1 RamA family antibiotic efflux transcriptional regulator -
FOB39_RS25960 5217147..5218262 - 1116 WP_012737592.1 MBL fold metallo-hydrolase -
FOB39_RS25965 5218442..5219023 + 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
FOB39_RS25970 5219023..5219391 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
FOB39_RS25975 5219511..5220164 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
FOB39_RS25980 5220429..5220716 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
FOB39_RS25985 5220709..5221026 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FOB39_RS25990 5221211..5222254 - 1044 WP_032419264.1 DUF2157 domain-containing protein -
FOB39_RS26000 5222921..5223787 - 867 WP_032419265.1 helix-turn-helix transcriptional regulator -
FOB39_RS26005 5223896..5225323 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T139905 WP_004142563.1 NZ_CP044033:c5221026-5220709 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T139905 NZ_CP058849:1767038-1767289 [Shigella flexneri]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT139905 WP_004142561.1 NZ_CP044033:c5220716-5220429 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT139905 NZ_CP058849:1767286-1767540 [Shigella flexneri]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References