Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3552403..3553073 Replicon chromosome
Accession NZ_CP043995
Organism Mycobacterium tuberculosis strain 4-0041P6C4

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag FPJ87_RS16660 Protein ID WP_003899954.1
Coordinates 3552403..3552747 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag FPJ87_RS16665 Protein ID WP_003899955.1
Coordinates 3552744..3553073 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ87_RS16630 3547476..3548336 + 861 WP_003416628.1 alpha/beta hydrolase -
FPJ87_RS16635 3548311..3548826 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
FPJ87_RS16640 3549066..3549359 + 294 WP_003416635.1 hypothetical protein -
FPJ87_RS16645 3549647..3550936 + 1290 WP_003906996.1 ATP-binding protein -
FPJ87_RS16650 3551283..3551717 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
FPJ87_RS16655 3551720..3552172 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
FPJ87_RS16660 3552403..3552747 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FPJ87_RS16665 3552744..3553073 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
FPJ87_RS16670 3553611..3553958 + 348 WP_003899956.1 DUF2384 domain-containing protein -
FPJ87_RS16675 3553955..3554575 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
FPJ87_RS16680 3554735..3556000 - 1266 WP_003902423.1 hypothetical protein -
FPJ87_RS16685 3556168..3556377 + 210 WP_003416778.1 hypothetical protein -
FPJ87_RS16690 3556624..3557658 - 1035 WP_003416786.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T139741 WP_003899954.1 NZ_CP043995:3552403-3552747 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T139741 NZ_CP058791:27583-28113 [Shigella flexneri]
ATGGACGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATGAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTCACCGATCATCTGAAACGTCAGCACAACAGTAAGATTTTACGTGGTTATCTGCTCGTGACGAGAGAGG
ATAAACCACGTGTCATGGGGTACTACACTCTGTCTGGCAGTTGTTTTGAGAAAACACTTCTACCCAGCAAAACACAACAA
AAGCGTGTGCCCTATAAAAATGTTCCTAGTGTGACTTTGGGCCGTCTGGCAATTGATAAAAATCTTCATCGCCAGGGATA
TGGAGAAACACTTGTTACTCATGCTATGAAAGTTGTGTATCAGGCCTCGCAAGCTGTGGGTATACACGGAATGTTCGTGG
AAGCACTGAACGACAACGCCAAAAAGTTCTACCTTCGAATGGGTTTTATACAGTTAAAGGAAGAAAACTGTAATTCGCTC
TTCTACCCGACAAAATCTATTGAAGAATTGTTCGAAGTAAATGATGAATAA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT139741 WP_003899955.1 NZ_CP043995:3552744-3553073 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT139741 NZ_CP058791:27310-27579 [Shigella flexneri]
ATGTCAGTACTGAAAAAGCAGCGCATAGATCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCAATCTCACAGTTTATGGTCAGTACCGCTTCAGAACGCGCTGCAGAAGTTATTGATCAGCACCGTCGAC
TGCTCCTTAATGAGGAGTCATGGAACCTGGTTATGGATGCCATCACTAATCCGCCAGTACCAAATGAACGACTGAAACGA
GCCGCTAAACGTCTCCGGGAACTGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References