Detailed information of TA system TA139741

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3552403..3553073
• Replicon: chromosome
• Accession: NZ_CP043995
• Organism: Mycobacterium tuberculosis strain 4-0041P6C4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: O53332
• Locus tag: FPJ87_RS16660
• Protein ID: WP_003899954.1
• Coordinates: 3552403..3552747 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: G0THF6
• Locus tag: FPJ87_RS16665
• Protein ID: WP_003899955.1
• Coordinates: 3552744..3553073 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ87_RS16630	3547476..3548336	+	861	WP_003416628.1	alpha/beta hydrolase	-
FPJ87_RS16635	3548311..3548826	+	516	WP_003899950.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
FPJ87_RS16640	3549066..3549359	+	294	WP_003416635.1	hypothetical protein	-
FPJ87_RS16645	3549647..3550936	+	1290	WP_003906996.1	ATP-binding protein	-
FPJ87_RS16650	3551283..3551717	-	435	WP_003899952.1	type II toxin-antitoxin system VapC family toxin	-
FPJ87_RS16655	3551720..3552172	-	453	WP_003899953.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FPJ87_RS16660	3552403..3552747	+	345	WP_003899954.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FPJ87_RS16665	3552744..3553073	+	330	WP_003899955.1	XRE family transcriptional regulator	Antitoxin
FPJ87_RS16670	3553611..3553958	+	348	WP_003899956.1	DUF2384 domain-containing protein	-
FPJ87_RS16675	3553955..3554575	+	621	WP_003899957.1	RES family NAD+ phosphorylase	-
FPJ87_RS16680	3554735..3556000	-	1266	WP_003902423.1	hypothetical protein	-
FPJ87_RS16685	3556168..3556377	+	210	WP_003416778.1	hypothetical protein	-
FPJ87_RS16690	3556624..3557658	-	1035	WP_003416786.1	IS30 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12692.50 Da
• Isoelectric Point: 5.6920

>T139741 WP_003899954.1 NZ_CP043995:3552403-3552747 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Nucleotide

• Length: 345 bp

>T139741 NZ_CP058791:27583-28113 [Shigella flexneri]
ATGGACGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATGAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTCACCGATCATCTGAAACGTCAGCACAACAGTAAGATTTTACGTGGTTATCTGCTCGTGACGAGAGAGG
ATAAACCACGTGTCATGGGGTACTACACTCTGTCTGGCAGTTGTTTTGAGAAAACACTTCTACCCAGCAAAACACAACAA
AAGCGTGTGCCCTATAAAAATGTTCCTAGTGTGACTTTGGGCCGTCTGGCAATTGATAAAAATCTTCATCGCCAGGGATA
TGGAGAAACACTTGTTACTCATGCTATGAAAGTTGTGTATCAGGCCTCGCAAGCTGTGGGTATACACGGAATGTTCGTGG
AAGCACTGAACGACAACGCCAAAAAGTTCTACCTTCGAATGGGTTTTATACAGTTAAAGGAAGAAAACTGTAATTCGCTC
TTCTACCCGACAAAATCTATTGAAGAATTGTTCGAAGTAAATGATGAATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11802.46 Da
• Isoelectric Point: 7.4051

>AT139741 WP_003899955.1 NZ_CP043995:3552744-3553073 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Nucleotide

• Length: 330 bp

>AT139741 NZ_CP058791:27310-27579 [Shigella flexneri]
ATGTCAGTACTGAAAAAGCAGCGCATAGATCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCAATCTCACAGTTTATGGTCAGTACCGCTTCAGAACGCGCTGCAGAAGTTATTGATCAGCACCGTCGAC
TGCTCCTTAATGAGGAGTCATGGAACCTGGTTATGGATGCCATCACTAATCCGCCAGTACCAAATGAACGACTGAAACGA
GCCGCTAAACGTCTCCGGGAACTGGAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U4FBK2

Antitoxin

• AlphaFold DB: G0THF6