Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2153866..2154785 Replicon chromosome
Accession NZ_CP043995
Organism Mycobacterium tuberculosis strain 4-0041P6C4

Toxin (Protein)


Gene name HigB2 Uniprot ID A0A654U351
Locus tag FPJ87_RS10190 Protein ID WP_003906719.1
Coordinates 2153866..2154471 (+) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag FPJ87_RS10195 Protein ID WP_003410124.1
Coordinates 2154480..2154785 (+) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ87_RS10170 2151354..2152250 + 897 WP_003906720.1 hypothetical protein -
FPJ87_RS10175 2152457..2152693 + 237 WP_003410133.1 hypothetical protein -
FPJ87_RS10180 2152645..2153322 + 678 WP_003900450.1 hypothetical protein -
FPJ87_RS10185 2153482..2153841 + 360 WP_003410131.1 hypothetical protein -
FPJ87_RS10190 2153866..2154471 + 606 WP_003906719.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FPJ87_RS10195 2154480..2154785 + 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
FPJ87_RS10200 2154870..2155169 + 300 WP_003410120.1 hypothetical protein -
FPJ87_RS10205 2155185..2155487 - 303 WP_003899135.1 hypothetical protein -
FPJ87_RS10215 2156949..2157668 - 720 WP_003410108.1 DUF433 domain-containing protein -
FPJ87_RS10220 2157910..2158950 - 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
FPJ87_RS10225 2158947..2159522 - 576 WP_003410100.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22716.91 Da        Isoelectric Point: 7.3073

>T139720 WP_003906719.1 NZ_CP043995:2153866-2154471 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLADTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T139720 NZ_CP058787:68613-68993 [Shigella flexneri]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACGGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGTATACAGGTATATGATTCTCCCGTGCTGGTGGAACTTGCGGTGGGGGCCGCAACTGG
TGAAATCCCCGTATCTTCAGTAGCGGAAAAACTACGTGAATTATTTGGTTCCAATATTTGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT139720 WP_003410124.1 NZ_CP043995:2154480-2154785 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT139720 NZ_CP058787:68392-68613 [Shigella flexneri]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGAGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTTGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A654U351

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References