Detailed information of TA system TA139667

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 106257..106682
• Replicon: plasmid pST95-32-2
• Accession: NZ_CP043952
• Organism: Escherichia coli strain ST95-32

Toxin (Protein)

• Gene name: hok
• Uniprot ID: A0A0D8W959
• Locus tag: F3J96_RS27155
• Protein ID: WP_001332444.1
• Coordinates: 106521..106682 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	106257..106468 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F3J96_RS27125	102231..102728	+	498	WP_050427786.1	single-stranded DNA-binding protein	-
F3J96_RS27130	102791..103024	+	234	WP_001209608.1	DUF905 domain-containing protein	-
F3J96_RS27135	103083..105041	+	1959	Protein_91	ParB/RepB/Spo0J family partition protein	-
F3J96_RS27140	105096..105530	+	435	WP_000845935.1	conjugation system SOS inhibitor PsiB	-
F3J96_RS27145	105527..106246	+	720	WP_001276275.1	plasmid SOS inhibition protein A	-
-	106257..106468	+	212	NuclAT_0	-	Antitoxin
-	106257..106468	+	212	NuclAT_0	-	Antitoxin
-	106257..106468	+	212	NuclAT_0	-	Antitoxin
-	106257..106468	+	212	NuclAT_0	-	Antitoxin
F3J96_RS27155	106521..106682	+	162	WP_001332444.1	type I toxin-antitoxin system Hok family toxin	Toxin
F3J96_RS27160	106920..107532	-	613	Protein_95	hypothetical protein	-
F3J96_RS27165	107711..108532	+	822	WP_001234485.1	DUF945 domain-containing protein	-
F3J96_RS27170	108828..109418	-	591	WP_000252676.1	transglycosylase SLT domain-containing protein	-
F3J96_RS27180	109752..110135	+	384	WP_000124981.1	relaxosome protein TraM	-
F3J96_RS27185	110325..111011	+	687	WP_000332490.1	PAS domain-containing protein	-
F3J96_RS27190	111105..111332	+	228	WP_001442099.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	vat / iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA	1..189994	189994
	-	flank	IS/Tn	-	-	100882..101385	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5976.19 Da
• Isoelectric Point: 8.5101

>T139667 WP_001332444.1 NZ_CP043952:106521-106682 [Escherichia coli]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTASLAYESSGK

Nucleotide

• Length: 162 bp

>T139667 NZ_CP058771:c4127977-4127690 [Shigella flexneri]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Nucleotide

• Length: 212 bp

>AT139667 NZ_CP043952:106257-106468 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTCTTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
AGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGC
AGAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGCCCCCTGTATGTCT

Structures

Toxin

• AlphaFold DB: A0A0D8W959

Antitoxin