Detailed information of TA system TA139647

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2972730..2972955
• Replicon: chromosome
• Accession: NZ_CP043950
• Organism: Escherichia coli strain ST95-32

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: F3J96_RS15085
• Protein ID: WP_000813254.1
• Coordinates: 2972730..2972885 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2972897..2972955 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F3J96_RS15030	2967810..2968124	-	315	WP_150330653.1	DUF1327 domain-containing protein	-
F3J96_RS15035	2968129..2968344	-	216	WP_000839572.1	class II holin family protein	-
F3J96_RS15060	2969069..2969782	-	714	WP_000342820.1	hypothetical protein	-
F3J96_RS15065	2970028..2970849	-	822	WP_001235237.1	antitermination protein	-
F3J96_RS15070	2970846..2971220	-	375	WP_001217427.1	RusA family crossover junction endodeoxyribonuclease	-
F3J96_RS15075	2971233..2972282	-	1050	WP_001265292.1	DUF968 domain-containing protein	-
F3J96_RS15080	2972284..2972562	-	279	WP_024193993.1	hypothetical protein	-
F3J96_RS15085	2972730..2972885	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
-	2972897..2972955	+	59	-	-	Antitoxin
F3J96_RS15095	2973123..2973788	-	666	WP_000150294.1	epoxyqueuosine reductase QueH	-
F3J96_RS15100	2973963..2974388	-	426	WP_001151152.1	DUF977 family protein	-
F3J96_RS15105	2974429..2975499	-	1071	WP_001262355.1	hypothetical protein	-
F3J96_RS15110	2975571..2975996	-	426	WP_000693867.1	Rha family transcriptional regulator	-
F3J96_RS15115	2975980..2976222	-	243	WP_000747951.1	hypothetical protein	-
F3J96_RS15120	2976614..2976952	+	339	WP_001362937.1	peptidase S24	-
F3J96_RS15125	2977245..2977400	+	156	WP_000379589.1	DUF1391 family protein	-
F3J96_RS15130	2977560..2977781	+	222	WP_001171970.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ybtS / ybtX / ybtQ / ybtP	2943465..2991606	48141

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T139647 WP_000813254.1 NZ_CP043950:c2972885-2972730 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T139647 NZ_CP058771:c71049-70672 [Shigella flexneri]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCAGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Nucleotide

• Length: 59 bp

>AT139647 NZ_CP043950:2972897-2972955 [Escherichia coli]
CCTTGCCTTTCGGCATGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin