Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 1298605..1299284 Replicon chromosome
Accession NZ_CP043950
Organism Escherichia coli strain ST95-32

Toxin (Protein)


Gene name higB Uniprot ID V0VBP6
Locus tag F3J96_RS06360 Protein ID WP_000057524.1
Coordinates 1298605..1298907 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag F3J96_RS06365 Protein ID WP_000806442.1
Coordinates 1298943..1299284 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F3J96_RS06335 1293981..1295633 + 1653 WP_000771762.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
F3J96_RS06340 1295671..1296174 - 504 WP_000667000.1 hypothetical protein -
F3J96_RS06345 1296171..1296971 - 801 WP_000439796.1 hypothetical protein -
F3J96_RS06350 1296995..1297474 - 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
F3J96_RS06355 1297678..1298472 - 795 WP_000365177.1 TraB/GumN family protein -
F3J96_RS06360 1298605..1298907 + 303 WP_000057524.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
F3J96_RS06365 1298943..1299284 + 342 WP_000806442.1 HigA family addiction module antidote protein Antitoxin
F3J96_RS06370 1299342..1301846 - 2505 WP_000083945.1 copper-exporting P-type ATPase CopA -
F3J96_RS06375 1302108..1303040 + 933 WP_000883052.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1296171..1307324 11153


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11839.47 Da        Isoelectric Point: 10.2237

>T139627 WP_000057524.1 NZ_CP043950:1298605-1298907 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHWDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T139627 NZ_CP058752:4795429-4795839 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT139627 WP_000806442.1 NZ_CP043950:1298943-1299284 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT139627 NZ_CP058752:4795182-4795448 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0VBP6

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References