Detailed information of TA system TA139625

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1069670..1070364
• Replicon: chromosome
• Accession: NZ_CP043950
• Organism: Escherichia coli strain ST95-32

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: F3J96_RS05290
• Protein ID: WP_001263500.1
• Coordinates: 1069966..1070364 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: F3J96_RS05285
• Protein ID: WP_000554758.1
• Coordinates: 1069670..1069963 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F3J96_RS05265	1065206..1065703	+	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-
F3J96_RS27730	1065786..1065944	-	159	WP_014639450.1	hypothetical protein	-
F3J96_RS05270	1066023..1067735	-	1713	Protein_990	flagellar biosynthesis protein FlhA	-
F3J96_RS05275	1067707..1068492	+	786	WP_000207544.1	putative lateral flagellar export/assembly protein LafU	-
F3J96_RS05280	1068563..1069618	+	1056	WP_001226168.1	DNA polymerase IV	-
F3J96_RS05285	1069670..1069963	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
F3J96_RS05290	1069966..1070364	+	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
F3J96_RS05295	1070374..1070826	+	453	WP_001059895.1	GNAT family N-acetyltransferase	-
F3J96_RS05300	1071016..1072155	+	1140	WP_000521577.1	RNA ligase RtcB family protein	-
F3J96_RS05305	1072152..1072766	+	615	WP_000602123.1	peptide chain release factor H	-
F3J96_RS05310	1072823..1074280	-	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
F3J96_RS05315	1074541..1074999	+	459	WP_001291988.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	1050145..1070826	20681

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T139625 WP_001263500.1 NZ_CP043950:1069966-1070364 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T139625 NZ_CP058752:4283338-4283685 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT139625 WP_000554758.1 NZ_CP043950:1069670-1069963 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT139625 NZ_CP058752:4283685-4283984 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGACTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATGGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3