Detailed information of TA system TA139612

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 49077..49503
• Replicon: plasmid pMPCMY-2
• Accession: NZ_CP043949
• Organism: Escherichia coli strain AR202.2

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: F3P13_RS25180
• Protein ID: WP_001312861.1
• Coordinates: 49077..49235 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	49279..49503 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F3P13_RS25130	44100..44327	-	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-
F3P13_RS25135	44464..45135	-	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
F3P13_RS25140	45329..45712	-	384	WP_001354030.1	relaxosome protein TraM	-
F3P13_RS25150	46035..46637	+	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
F3P13_RS25155	46934..47755	-	822	WP_001234445.1	DUF945 domain-containing protein	-
F3P13_RS25160	47866..48162	-	297	WP_001272251.1	hypothetical protein	-
F3P13_RS25535	48462..48839	+	378	Protein_60	hypothetical protein	-
F3P13_RS25180	49077..49235	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	49279..49503	-	225	NuclAT_0	-	Antitoxin
-	49279..49503	-	225	NuclAT_0	-	Antitoxin
-	49279..49503	-	225	NuclAT_0	-	Antitoxin
-	49279..49503	-	225	NuclAT_0	-	Antitoxin
F3P13_RS25540	49315..49494	+	180	WP_001309233.1	hypothetical protein	-
F3P13_RS25185	49515..50234	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
F3P13_RS25190	50231..50665	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
F3P13_RS25195	50734..52757	-	2024	Protein_65	ParB/RepB/Spo0J family partition protein	-
F3P13_RS25200	52818..53051	-	234	WP_000006003.1	DUF905 family protein	-
F3P13_RS25205	53109..53636	-	528	WP_000290834.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	erm(B) / blaCMY-2	-	1..66611	66611

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T139612 WP_001312861.1 NZ_CP043949:c49235-49077 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T139612 NZ_CP058749:95420-95725 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT139612 NZ_CP043949:c49503-49279 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin