Detailed information of TA system TA139608

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 44410..44664
• Replicon: plasmid pMPNDM-5
• Accession: NZ_CP043948
• Organism: Escherichia coli strain AR202.2

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: F3P13_RS24480
• Protein ID: WP_015387399.1
• Coordinates: 44458..44664 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	44410..44471 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F3P13_RS24435	40062..40274	+	213	WP_005012601.1	hypothetical protein	-
F3P13_RS25515	40575..40664	-	90	Protein_45	IS1 family transposase	-
F3P13_RS24450	40719..41396	+	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
F3P13_RS24455	41396..41743	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
F3P13_RS24460	41763..43334	+	1572	WP_023149734.1	IS66 family transposase	-
F3P13_RS24465	43372..43988	-	617	Protein_49	IS1 family transposase	-
F3P13_RS24470	44089..44271	+	183	WP_000968309.1	hypothetical protein	-
-	44410..44471	-	62	NuclAT_0	-	Antitoxin
-	44410..44471	-	62	NuclAT_0	-	Antitoxin
-	44410..44471	-	62	NuclAT_0	-	Antitoxin
-	44410..44471	-	62	NuclAT_0	-	Antitoxin
F3P13_RS24480	44458..44664	+	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
F3P13_RS24485	44948..45205	+	258	WP_000083833.1	replication regulatory protein RepA	-
F3P13_RS24495	45441..45515	+	75	WP_032336874.1	RepA leader peptide Tap	-
F3P13_RS24500	45508..45954	+	447	Protein_54	incFII family plasmid replication initiator RepA	-
F3P13_RS24505	45900..46382	-	483	Protein_55	VENN motif pre-toxin domain-containing protein	-
F3P13_RS24510	47275..48495	+	1221	WP_000410951.1	arginine deiminase	-
F3P13_RS24515	48506..49417	+	912	WP_000440183.1	carbamate kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sitABCD / tet(A) / dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 / aac(3)-IIa / mph(A)	iucA / iucB / iucC / iucD / iutA	1..99454	99454

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T139608 WP_015387399.1 NZ_CP043948:44458-44664 [Escherichia coli]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T139608 NZ_CP058748:c4233694-4233344 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATTTACGCGCCCGACTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT139608 NZ_CP043948:c44471-44410 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin