Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 8118..8382 Replicon plasmid pMPTEM-30
Accession NZ_CP043947
Organism Escherichia coli strain AR202.2

Toxin (Protein)


Gene name pndA Uniprot ID E6BRV3
Locus tag F3P13_RS23440 Protein ID WP_001303307.1
Coordinates 8230..8382 (+) Length 51 a.a.

Antitoxin (RNA)


Gene name pndB
Locus tag -
Coordinates 8118..8180 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F3P13_RS23425 3357..5648 - 2292 WP_001289275.1 hypothetical protein -
F3P13_RS23430 5641..6711 - 1071 WP_000151576.1 IncI1-type conjugal transfer protein TrbB -
F3P13_RS23435 6730..7938 - 1209 WP_000121273.1 IncI1-type conjugal transfer protein TrbA -
- 8118..8180 - 63 NuclAT_0 - Antitoxin
- 8118..8180 - 63 NuclAT_0 - Antitoxin
- 8118..8180 - 63 NuclAT_0 - Antitoxin
- 8118..8180 - 63 NuclAT_0 - Antitoxin
F3P13_RS23440 8230..8382 + 153 WP_001303307.1 Hok/Gef family protein Toxin
F3P13_RS23445 8454..8705 - 252 WP_001291965.1 hypothetical protein -
F3P13_RS25495 9365..9502 - 138 WP_047088497.1 hypothetical protein -
F3P13_RS23450 9547..10773 - 1227 WP_024186316.1 transposase -
F3P13_RS23455 10832..11236 + 405 WP_001175009.1 IS200/IS605 family transposase -
F3P13_RS23460 11568..11777 - 210 WP_032178830.1 hemolysin expression modulator Hha -
F3P13_RS23465 11875..12537 - 663 WP_000644794.1 plasmid IncI1-type surface exclusion protein ExcA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul2 / floR / blaTEM-30 / sul1 / qacE / ant(3'')-Ia / dfrA1 - 1..115932 115932


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(7-46)


Sequences

Toxin        


Download         Length: 51 a.a.        Molecular weight: 5817.21 Da        Isoelectric Point: 8.7948

>T139601 WP_001303307.1 NZ_CP043947:8230-8382 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Download         Length: 153 bp

>T139601 NZ_CP058748:c2710912-2710810 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 63 bp

>AT139601 NZ_CP043947:c8180-8118 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A634Q5L1

Antitoxin

Download structure file

References