Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3791460..3792001 Replicon chromosome
Accession NZ_CP043946
Organism Escherichia coli strain AR202.2

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag F3P13_RS18400 Protein ID WP_000615983.1
Coordinates 3791723..3792001 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag F3P13_RS18395 Protein ID WP_000729703.1
Coordinates 3791460..3791720 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F3P13_RS18375 3787135..3787920 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
F3P13_RS18380 3787865..3789604 + 1740 WP_001334802.1 flagellar biosynthesis protein FlhA -
F3P13_RS18385 3789828..3790325 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
F3P13_RS18390 3790501..3791250 - 750 WP_000056849.1 C40 family peptidase -
F3P13_RS18395 3791460..3791720 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
F3P13_RS18400 3791723..3792001 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
F3P13_RS18405 3792157..3792897 + 741 WP_001225679.1 murein L,D-transpeptidase -
F3P13_RS18410 3792868..3793635 - 768 WP_000333380.1 class II glutamine amidotransferase -
F3P13_RS18415 3793841..3794419 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T139597 WP_000615983.1 NZ_CP043946:3791723-3792001 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T139597 NZ_CP058748:c2102971-2102864 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT139597 WP_000729703.1 NZ_CP043946:3791460-3791720 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT139597 NZ_CP058748:2103019-2103085 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTAAACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References