Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1907589..1908091 Replicon chromosome
Accession NZ_CP043946
Organism Escherichia coli strain AR202.2

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag F3P13_RS09170 Protein ID WP_000767829.1
Coordinates 1907837..1908091 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag F3P13_RS09165 Protein ID WP_001259255.1
Coordinates 1907589..1907840 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F3P13_RS09140 1902766..1903833 - 1068 WP_000080090.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
F3P13_RS09145 1903833..1904903 - 1071 WP_000108933.1 histidinol-phosphate transaminase -
F3P13_RS09150 1904900..1906204 - 1305 WP_074514295.1 histidinol dehydrogenase -
F3P13_RS09155 1906210..1907109 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
F3P13_RS09160 1907255..1907305 - 51 WP_001364200.1 his operon leader peptide -
F3P13_RS09165 1907589..1907840 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
F3P13_RS09170 1907837..1908091 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
F3P13_RS09175 1908174..1908998 + 825 WP_000754737.1 SDR family oxidoreductase -
F3P13_RS09180 1909044..1909973 + 930 WP_000803366.1 LysR family transcriptional regulator -
F3P13_RS09185 1910188..1910250 + 63 WP_010723108.1 membrane protein YoeI -
F3P13_RS09190 1910240..1911598 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
F3P13_RS09195 1911777..1912835 + 1059 WP_087523589.1 transport protein YeeE -
F3P13_RS09200 1912849..1913076 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T139583 WP_000767829.1 NZ_CP043946:1907837-1908091 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T139583 NZ_CP058743:c5248173-5247790 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTACCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT139583 WP_001259255.1 NZ_CP043946:1907589-1907840 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT139583 NZ_CP058743:c5248415-5248173 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References