Detailed information of TA system TA139567

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 59148..59402
• Replicon: plasmid pMPNDM-5
• Accession: NZ_CP043944
• Organism: Escherichia coli strain AR216.2b

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: F3P14_RS24570
• Protein ID: WP_015387399.1
• Coordinates: 59148..59354 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	59341..59402 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F3P14_RS24535	54395..55306	-	912	WP_000440183.1	carbamate kinase	-
F3P14_RS24540	55317..56537	-	1221	WP_000410951.1	arginine deiminase	-
F3P14_RS24545	57430..57912	+	483	Protein_66	VENN motif pre-toxin domain-containing protein	-
F3P14_RS24550	57858..58304	-	447	Protein_67	incFII family plasmid replication initiator RepA	-
F3P14_RS24555	58297..58371	-	75	WP_032336874.1	RepA leader peptide Tap	-
F3P14_RS24565	58607..58864	-	258	WP_000083833.1	replication regulatory protein RepA	-
F3P14_RS24570	59148..59354	-	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	59341..59402	+	62	NuclAT_0	-	Antitoxin
-	59341..59402	+	62	NuclAT_0	-	Antitoxin
-	59341..59402	+	62	NuclAT_0	-	Antitoxin
-	59341..59402	+	62	NuclAT_0	-	Antitoxin
F3P14_RS24580	59541..59723	-	183	WP_000968309.1	hypothetical protein	-
F3P14_RS24585	59824..60440	+	617	Protein_72	IS1 family transposase	-
F3P14_RS24590	60478..62049	-	1572	WP_023149734.1	IS66 family transposase	-
F3P14_RS24595	62069..62416	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
F3P14_RS24600	62416..63093	-	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
F3P14_RS25510	63148..63237	+	90	Protein_76	IS1 family transposase	-
F3P14_RS24615	63538..63750	-	213	WP_005012601.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mph(A) / aac(3)-IIa / blaNDM-5 / sul1 / qacE / aadA2 / dfrA12 / tet(A) / sitABCD	iutA / iucD / iucC / iucB / iucA	1..99465	99465

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T139567 WP_015387399.1 NZ_CP043944:c59354-59148 [Escherichia coli]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T139567 NZ_CP058742:597315-597584 [Streptococcus suis]
ATGGCTTATAAGCTTGTTCTCAGCGATGATGCTTTGAAGCAGTTGAAAAAAATGGACAGGCATGTGGGGATGATGCTTGC
TAAGGATTTGAAAAAACGTTTGGACGGTTTGGAGAATCCAAGACAATTTGGGAAGGCCTTGGTAGGAGACTATAAGGGGC
TGTGGCGGTATAGAGTGGGGAATTACCGTGTAATTTGTGATATTATTGATAACAAAATGGTCATCCTGGCCCTTGAAATC
GGGCACCGGAAAGAAATCTATAAAAAATGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT139567 NZ_CP043944:59341-59402 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin