Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3785463..3786157 Replicon chromosome
Accession NZ_CP043942
Organism Escherichia coli strain AR216.2b

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag F3P14_RS18360 Protein ID WP_001263489.1
Coordinates 3785463..3785861 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag F3P14_RS18365 Protein ID WP_000554758.1
Coordinates 3785864..3786157 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 3781051..3781131 - 81 NuclAT_12 - -
- 3781051..3781131 - 81 NuclAT_12 - -
- 3781051..3781131 - 81 NuclAT_12 - -
- 3781051..3781131 - 81 NuclAT_12 - -
F3P14_RS18335 3781727..3782185 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
F3P14_RS18340 3782446..3783903 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
F3P14_RS18345 3783960..3784481 - 522 Protein_3538 peptide chain release factor H -
F3P14_RS18350 3784477..3784683 - 207 Protein_3539 RtcB family protein -
F3P14_RS18355 3785001..3785453 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
F3P14_RS18360 3785463..3785861 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
F3P14_RS18365 3785864..3786157 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
F3P14_RS18370 3786209..3787264 - 1056 WP_001226164.1 DNA polymerase IV -
F3P14_RS18375 3787335..3788120 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
F3P14_RS18380 3788065..3789804 + 1740 WP_001334802.1 flagellar biosynthesis protein FlhA -
F3P14_RS18385 3790028..3790525 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T139557 WP_001263489.1 NZ_CP043942:c3785861-3785463 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T139557 NZ_CP058731:6956-7312 [Acinetobacter baumannii]
ATGTGGACAGTCATTACAACGGATCTGTTTAACGAATGGCTTGTACAGCAAGATGAATCTACACAAGAGAAAGTACTCGC
AGCGTTGGTCATTCTACAGCAACAGGGACCAAGTTTAGGTCGTCCTTTAGTAGATACCGTCTATGACTCCAAATTTACCA
ACATGAAAGAGCTACGTGTTCAGCATCGAGGTAAACCACTGAGAGCCTTTTTCGCATTTGATCCTTTACGACAAGCTATT
GTGCTGTGTATCGGTGATAAAGGCGGTAAAAAGCGTTTTTATAAAGAGATGTTAGATATTGCCGATCAGCAATACGAACT
TCATCTTTCAACTTTAGGAGATCAATCTAATGGCTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT139557 WP_000554758.1 NZ_CP043942:c3786157-3785864 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT139557 NZ_CP058731:7305-7607 [Acinetobacter baumannii]
ATGGCTAAGACCCTACAAGAATTATTGGCTAGTCGCTCTCCAGAGAGTCAAGCTCGTATTCAAAAAATGGCTGATGAATT
ACTGTTGGAAACTCAGCTTCACCTTATTCGTGAAGAGTTAGAGATTTCTCAAAAAGAACTGGCTGCAACCCTTGGAATCA
AACAGCCGTCATTGTCAGCAATCGAAAATCGTGGTCATGATCTTAAAATTTCAACGATGAAAAAGTATGTTGAAGCAATG
GGCGGAAAACTTCGTATTGATGTAGAGCTTCCAACAGGAAAACACATAGGATTTAATGTCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References