Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1653414..1653976 Replicon chromosome
Accession NZ_CP043937
Organism Escherichia coli strain RM14516

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag FS841_RS07940 Protein ID WP_000605676.1
Coordinates 1653698..1653976 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag FS841_RS07935 Protein ID WP_000781370.1
Coordinates 1653414..1653698 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FS841_RS07920 (1648596) 1648596..1651643 + 3048 WP_012602782.1 formate dehydrogenase-N subunit alpha -
FS841_RS07925 (1651656) 1651656..1652540 + 885 WP_001240565.1 formate dehydrogenase N subunit beta -
FS841_RS07930 (1652533) 1652533..1653186 + 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
FS841_RS07935 (1653414) 1653414..1653698 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
FS841_RS07940 (1653698) 1653698..1653976 - 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FS841_RS07945 (1654162) 1654162..1655172 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
FS841_RS07950 (1655306) 1655306..1657003 - 1698 WP_000433464.1 malate dehydrogenase -
FS841_RS07955 (1657160) 1657160..1657297 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
FS841_RS07960 (1657399) 1657399..1657614 - 216 WP_000495766.1 biofilm-dependent modulation protein -
FS841_RS07965 (1657959) 1657959..1658390 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T139518 WP_000605676.1 NZ_CP043937:c1653976-1653698 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T139518 NZ_CP058719:c923426-923319 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT139518 WP_000781370.1 NZ_CP043937:c1653698-1653414 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT139518 NZ_CP058719:923474-923540 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References