Detailed information of TA system TA139503

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 95406..95832
• Replicon: plasmid pSCKLB555-3
• Accession: NZ_CP043935
• Organism: Klebsiella pneumoniae strain 555

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: F2X40_RS28935
• Protein ID: WP_001312861.1
• Coordinates: 95406..95564 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	95608..95832 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F2X40_RS28890	90461..90688	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
F2X40_RS28895	90782..91468	-	687	WP_015059009.1	PAS domain-containing protein	-
F2X40_RS28900	91659..92042	-	384	WP_000124981.1	relaxosome protein TraM	-
F2X40_RS28905	92319..92966	+	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
F2X40_RS28910	93263..94084	-	822	WP_001234445.1	DUF945 domain-containing protein	-
F2X40_RS28915	94195..94491	-	297	WP_001272251.1	hypothetical protein	-
F2X40_RS30630	94791..95168	+	378	Protein_113	hypothetical protein	-
F2X40_RS28935	95406..95564	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	95608..95832	-	225	NuclAT_0	-	Antitoxin
-	95608..95832	-	225	NuclAT_0	-	Antitoxin
-	95608..95832	-	225	NuclAT_0	-	Antitoxin
-	95608..95832	-	225	NuclAT_0	-	Antitoxin
F2X40_RS28940	95844..96563	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
F2X40_RS28945	96560..96994	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
F2X40_RS28950	97063..99086	-	2024	Protein_117	ParB/RepB/Spo0J family partition protein	-
F2X40_RS28955	99147..99380	-	234	WP_000006003.1	DUF905 family protein	-
F2X40_RS28960	99438..99965	-	528	WP_000290834.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-IIa / oqxB / oqxA / sul2 / tet(A) / floR / blaCTX-M-55 / blaTEM-1B / fosA3 / aph(3'')-Ib / aph(6)-Id	-	1..179179	179179

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T139503 WP_001312861.1 NZ_CP043935:c95564-95406 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T139503 NZ_CP058714:c4097991-4097884 [Escherichia coli]
ATGACGCTCACGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT139503 NZ_CP043935:c95832-95608 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin