Detailed information of TA system TA139499

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 55117..55370
• Replicon: plasmid pSCKLB555-3
• Accession: NZ_CP043935
• Organism: Klebsiella pneumoniae strain 555

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: F2X40_RS28680
• Protein ID: WP_001312851.1
• Coordinates: 55117..55266 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	55311..55370 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F2X40_RS28635	50476..50891	-	416	Protein_59	IS1 family transposase	-
F2X40_RS28640	51140..51541	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
F2X40_RS28645	51474..51731	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
F2X40_RS28650	51824..52477	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
F2X40_RS28660	53416..54273	-	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
F2X40_RS28665	54266..54340	-	75	WP_001375168.1	RepA leader peptide Tap	-
F2X40_RS28675	54585..54833	-	249	WP_000083837.1	replication regulatory protein RepA	-
F2X40_RS28680	55117..55266	-	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	55311..55370	+	60	NuclAT_1	-	Antitoxin
-	55311..55370	+	60	NuclAT_1	-	Antitoxin
-	55311..55370	+	60	NuclAT_1	-	Antitoxin
-	55311..55370	+	60	NuclAT_1	-	Antitoxin
F2X40_RS28690	55571..55801	-	231	WP_001736714.1	hypothetical protein	-
F2X40_RS28695	55965..56564	-	600	WP_032083981.1	hypothetical protein	-
F2X40_RS28700	56950..57150	-	201	WP_015059022.1	hypothetical protein	-
F2X40_RS28705	57282..57842	-	561	WP_000139328.1	fertility inhibition protein FinO	-
F2X40_RS28710	57897..58643	-	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-IIa / oqxB / oqxA / sul2 / tet(A) / floR / blaCTX-M-55 / blaTEM-1B / fosA3 / aph(3'')-Ib / aph(6)-Id	-	1..179179	179179
	-	inside	IScluster/Tn	aph(3')-IIa / oqxB / oqxA / sul2 / tet(A) / floR	-	15693..50891	35198

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T139499 WP_001312851.1 NZ_CP043935:c55266-55117 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T139499 NZ_CP058714:c2871907-2871533 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Nucleotide

• Length: 60 bp

>AT139499 NZ_CP043935:55311-55370 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin