Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 105453..106180 Replicon plasmid pSCKLB555-2
Accession NZ_CP043934
Organism Klebsiella pneumoniae strain 555

Toxin (Protein)


Gene name higB Uniprot ID A0A7W3D9W1
Locus tag F2X40_RS27965 Protein ID WP_011251285.1
Coordinates 105453..105764 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag F2X40_RS27970 Protein ID WP_011251286.1
Coordinates 105761..106180 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F2X40_RS27935 100684..101664 + 981 WP_048293850.1 IS5-like element ISKpn26 family transposase -
F2X40_RS27940 102209..102388 - 180 Protein_98 IS5/IS1182 family transposase -
F2X40_RS27945 102428..103966 - 1539 WP_032430751.1 IS66 family transposase -
F2X40_RS27950 104015..104362 - 348 WP_032430752.1 IS66 family insertion sequence element accessory protein TnpB -
F2X40_RS27955 104359..104697 - 339 WP_023288266.1 IS66 family insertion sequence hypothetical protein -
F2X40_RS27960 104790..105248 + 459 Protein_102 DDE-type integrase/transposase/recombinase -
F2X40_RS27965 105453..105764 + 312 WP_011251285.1 type II toxin-antitoxin system HigB family toxin Toxin
F2X40_RS27970 105761..106180 + 420 WP_011251286.1 helix-turn-helix domain-containing protein Antitoxin
F2X40_RS27975 106351..107316 - 966 Protein_105 IS5 family transposase -
F2X40_RS27980 107863..108432 + 570 WP_064160296.1 type 1 fimbrial protein -
F2X40_RS27985 108491..109186 + 696 WP_064160295.1 molecular chaperone -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..172565 172565
- inside IScluster/Tn - - 100684..107316 6632


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12346.12 Da        Isoelectric Point: 9.7248

>T139495 WP_011251285.1 NZ_CP043934:105453-105764 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Download         Length: 312 bp

>T139495 NZ_CP058714:2233163-2233270 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15377.46 Da        Isoelectric Point: 4.4420

>AT139495 WP_011251286.1 NZ_CP043934:105761-106180 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT139495 NZ_CP058714:c2233114-2233051 [Escherichia coli]
GTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W3D9W1

Antitoxin

Source ID Structure

References