Detailed information of TA system TA139400

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4680578..4681272
• Replicon: chromosome
• Accession: NZ_CP043852
• Organism: Escherichia coli ATCC 8739

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: F1719_RS22870
• Protein ID: WP_001263493.1
• Coordinates: 4680874..4681272 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: F1719_RS22865
• Protein ID: WP_000554757.1
• Coordinates: 4680578..4680871 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F1719_RS22845	4676218..4676715	+	498	WP_000006260.1	REP-associated tyrosine transposase RayT	-
F1719_RS22850	4676931..4678643	-	1713	Protein_4335	flagellar biosynthesis protein FlhA	-
F1719_RS22855	4678615..4679400	+	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
F1719_RS22860	4679471..4680526	+	1056	WP_001226164.1	DNA polymerase IV	-
F1719_RS22865	4680578..4680871	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
F1719_RS22870	4680874..4681272	+	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
F1719_RS22875	4681282..4681734	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
F1719_RS22880	4681980..4682183	+	204	Protein_4341	RNA ligase RtcB family protein	-
F1719_RS22885	4682182..4682703	+	522	Protein_4342	peptide chain release factor H	-
F1719_RS22890	4682760..4684217	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
F1719_RS22895	4684478..4684936	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
F1719_RS22900	4685028..4686272	+	1245	WP_000189541.1	esterase FrsA	-
-	4685532..4685612	+	81	NuclAT_11	-	-
-	4685532..4685612	+	81	NuclAT_11	-	-
-	4685532..4685612	+	81	NuclAT_11	-	-
-	4685532..4685612	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	4664236..4682703	18467

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T139400 WP_001263493.1 NZ_CP043852:4680874-4681272 [Escherichia coli ATCC 8739]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T139400 NZ_CP058663:126963-127379 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT139400 WP_000554757.1 NZ_CP043852:4680578-4680871 [Escherichia coli ATCC 8739]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT139400 NZ_CP058663:126736-126966 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6