Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4674542..4675083 Replicon chromosome
Accession NZ_CP043852
Organism Escherichia coli ATCC 8739

Toxin (Protein)


Gene name yafQ Uniprot ID A0A140NAP5
Locus tag F1719_RS22830 Protein ID WP_000615981.1
Coordinates 4674542..4674820 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag F1719_RS22835 Protein ID WP_000729704.1
Coordinates 4674823..4675083 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F1719_RS22815 4672125..4672703 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
F1719_RS22820 4672908..4673675 + 768 WP_000333379.1 class II glutamine amidotransferase -
F1719_RS22825 4673646..4674386 - 741 WP_001225679.1 murein L,D-transpeptidase -
F1719_RS22830 4674542..4674820 - 279 WP_000615981.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
F1719_RS22835 4674823..4675083 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
F1719_RS22840 4675269..4676042 + 774 WP_001353765.1 C40 family peptidase -
F1719_RS22845 4676218..4676715 + 498 WP_000006260.1 REP-associated tyrosine transposase RayT -
F1719_RS22850 4676931..4678643 - 1713 Protein_4335 flagellar biosynthesis protein FlhA -
F1719_RS22855 4678615..4679400 + 786 WP_000207564.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 4664236..4682703 18467


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10860.65 Da        Isoelectric Point: 10.0702

>T139399 WP_000615981.1 NZ_CP043852:c4674820-4674542 [Escherichia coli ATCC 8739]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQSSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T139399 NZ_CP058663:c125956-125651 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT139399 WP_000729704.1 NZ_CP043852:c4675083-4674823 [Escherichia coli ATCC 8739]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT139399 NZ_CP058663:c126176-125958 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MMG
PDB 4MMJ
PDB 4ML2
PDB 4ML0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References