Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2930486..2931213 Replicon chromosome
Accession NZ_CP043852
Organism Escherichia coli ATCC 8739

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag F1719_RS14590 Protein ID WP_000550189.1
Coordinates 2930899..2931213 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag F1719_RS14585 Protein ID WP_000560266.1
Coordinates 2930486..2930902 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F1719_RS14575 2925646..2927997 + 2352 WP_000695502.1 alpha-glucosidase -
F1719_RS14580 2928423..2930441 + 2019 WP_000121449.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
F1719_RS14585 2930486..2930902 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
F1719_RS14590 2930899..2931213 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
F1719_RS14595 2931497..2932633 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
F1719_RS14600 2932718..2933221 + 504 WP_001300832.1 M48 family metallopeptidase -
F1719_RS14605 2933298..2933990 + 693 WP_000942546.1 vancomycin high temperature exclusion protein -
F1719_RS14610 2934069..2935055 + 987 WP_001301393.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T139396 WP_000550189.1 NZ_CP043852:c2931213-2930899 [Escherichia coli ATCC 8739]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T139396 NZ_CP058662:c63193-63041 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT139396 WP_000560266.1 NZ_CP043852:c2930902-2930486 [Escherichia coli ATCC 8739]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT139396 NZ_CP058662:63245-63305 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References