Detailed information of TA system TA139395

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 2864503..2865196
• Replicon: chromosome
• Accession: NZ_CP043852
• Organism: Escherichia coli ATCC 8739

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: F1719_RS14280
• Protein ID: WP_000415584.1
• Coordinates: 2864900..2865196 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: F1719_RS14275
• Protein ID: WP_000650107.1
• Coordinates: 2864503..2864898 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F1719_RS14265	2860367..2862625	-	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-
F1719_RS14270	2862763..2864370	-	1608	WP_001329417.1	ABC transporter substrate-binding protein	-
F1719_RS14275	2864503..2864898	-	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
F1719_RS14280	2864900..2865196	-	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
F1719_RS14285	2865401..2865883	-	483	WP_000183505.1	transcriptional regulator	-
F1719_RS14290	2865936..2866328	-	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
F1719_RS14295	2866479..2867138	+	660	WP_001221493.1	two-component system response regulator QseB	-
F1719_RS14300	2867135..2868484	+	1350	WP_000673398.1	two-component system sensor histidine kinase QseC	-
F1719_RS14305	2868530..2868862	-	333	WP_000917684.1	DUF2645 family protein	-
F1719_RS14310	2869181..2869762	+	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
F1719_RS14315	2869793..2870107	+	315	WP_000958598.1	antibiotic biosynthesis monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T139395 WP_000415584.1 NZ_CP043852:c2865196-2864900 [Escherichia coli ATCC 8739]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T139395 NZ_CP058661:c4823461-4822997 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAACCCCATTGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT139395 WP_000650107.1 NZ_CP043852:c2864898-2864503 [Escherichia coli ATCC 8739]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT139395 NZ_CP058661:c4823796-4823461 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• PDB: 3HI2
• PDB: 3GA8
• PDB: 3O9X
• PDB: 2KZ8
• PDB: 3FMY
• PDB: 3GN5
• AlphaFold DB: A0A7U9LU65