Detailed information of TA system TA139378

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 994504..994875
• Replicon: chromosome
• Accession: NZ_CP043852
• Organism: Escherichia coli ATCC 8739

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: F1719_RS05385
• Protein ID: WP_001317028.1
• Coordinates: 994681..994875 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	994504..994682 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F1719_RS05360	990459..991832	+	1374	WP_000123737.1	ATP-dependent RNA helicase DbpA	-
F1719_RS05365	991961..992896	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
F1719_RS05370	992948..994183	-	1236	WP_000040852.1	site-specific integrase	-
F1719_RS05375	994185..994400	-	216	WP_000079604.1	excisionase XisR	-
F1719_RS05380	994479..994688	-	210	WP_073984280.1	double-strand break reduction protein RcbA	-
-	994504..994682	+	179	NuclAT_0	-	Antitoxin
-	994504..994682	+	179	NuclAT_0	-	Antitoxin
-	994504..994682	+	179	NuclAT_0	-	Antitoxin
-	994504..994682	+	179	NuclAT_0	-	Antitoxin
F1719_RS05385	994681..994875	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
F1719_RS05390	994932..995741	-	810	WP_073984275.1	recombination protein RecT	-
F1719_RS05395	995734..998334	-	2601	WP_203448695.1	exodeoxyribonuclease VIII	-
F1719_RS05400	998436..998711	-	276	WP_000632297.1	protein RacC	-
F1719_RS05405	998786..998956	-	171	WP_001352098.1	conserved protein, Rac prophage	-
F1719_RS05410	998956..999177	-	222	WP_000560225.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	985689..1023251	37562

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T139378 WP_001317028.1 NZ_CP043852:c994875-994681 [Escherichia coli ATCC 8739]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T139378 NZ_CP058661:1342767-1343117 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT139378 NZ_CP043852:994504-994682 [Escherichia coli ATCC 8739]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATTTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin