Detailed information of TA system TA139371

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 26996..27250
• Replicon: plasmid unnamed
• Accession: NZ_CP043851
• Organism: Escherichia coli ATCC 8739

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: F1719_RS00170
• Protein ID: WP_001351576.1
• Coordinates: 27044..27250 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	26996..27057 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F1719_RS00145	23744..24490	+	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-
F1719_RS00150	24549..25409	+	861	WP_000704513.1	alpha/beta hydrolase	-
F1719_RS00155	25512..26072	+	561	WP_000139315.1	fertility inhibition protein FinO	-
F1719_RS00160	26208..26420	+	213	WP_001299730.1	ANR family transcriptional regulator	-
F1719_RS00165	26666..26740	+	75	Protein_32	endonuclease	-
-	26996..27057	-	62	NuclAT_1	-	Antitoxin
-	26996..27057	-	62	NuclAT_1	-	Antitoxin
-	26996..27057	-	62	NuclAT_1	-	Antitoxin
-	26996..27057	-	62	NuclAT_1	-	Antitoxin
F1719_RS00170	27044..27250	+	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
F1719_RS00175	27534..27791	+	258	WP_000083845.1	replication regulatory protein RepA	-
F1719_RS00180	28023..28097	+	75	WP_001442103.1	RepA leader peptide Tap	-
F1719_RS00185	28090..28572	+	483	WP_001273588.1	hypothetical protein	-
F1719_RS00190	28565..29422	+	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
F1719_RS00195	30358..30702	+	345	WP_001553768.1	hypothetical protein	-
F1719_RS00200	30883..31998	-	1116	Protein_39	IS91 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	vat / iroN / iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA	1..116228	116228

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T139371 WP_001351576.1 NZ_CP043851:27044-27250 [Escherichia coli ATCC 8739]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T139371 NZ_CP058658:100032-100448 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT139371 NZ_CP043851:c27057-26996 [Escherichia coli ATCC 8739]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin