Detailed information of TA system TA139330

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 44088..44514
• Replicon: plasmid p3RM8082
• Accession: NZ_CP043827
• Organism: Escherichia coli strain RM8082

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: F1745_RS26020
• Protein ID: WP_096937776.1
• Coordinates: 44088..44213 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	44290..44514 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F1745_RS25995 (40262)	40262..40789	+	528	WP_000182867.1	hypothetical protein	-
F1745_RS26000 (40831)	40831..40991	-	161	Protein_45	transposase domain-containing protein	-
F1745_RS26005 (41114)	41114..42684	-	1571	Protein_46	IS66-like element ISCro1 family transposase	-
F1745_RS26010 (42704)	42704..43051	-	348	WP_024244069.1	IS66 family insertion sequence element accessory protein TnpB	-
F1745_RS26015 (43051)	43051..43701	-	651	WP_069906978.1	IS66-like element accessory protein TnpA	-
F1745_RS26020 (44088)	44088..44213	-	126	WP_096937776.1	type I toxin-antitoxin system Hok family toxin	Toxin
F1745_RS26025 (44155)	44155..44304	-	150	Protein_50	plasmid maintenance protein Mok	-
- (44292)	44292..44357	-	66	NuclAT_0	-	-
- (44292)	44292..44357	-	66	NuclAT_0	-	-
- (44292)	44292..44357	-	66	NuclAT_2	-	-
- (44295)	44295..44358	+	64	NuclAT_3	-	-
- (44295)	44295..44358	+	64	NuclAT_3	-	-
- (44290)	44290..44514	+	225	NuclAT_0	-	-
- (44290)	44290..44514	-	225	NuclAT_0	-	Antitoxin
- (44290)	44290..44514	-	225	NuclAT_0	-	Antitoxin
- (44290)	44290..44514	-	225	NuclAT_0	-	Antitoxin
- (44290)	44290..44514	-	225	NuclAT_0	-	Antitoxin
F1745_RS26030 (44326)	44326..44514	+	189	WP_032189914.1	hypothetical protein	-
F1745_RS26035 (44459)	44459..45245	-	787	Protein_52	plasmid SOS inhibition protein A	-
F1745_RS26040 (45242)	45242..45676	-	435	WP_000845915.1	conjugation system SOS inhibitor PsiB	-
F1745_RS26045 (45745)	45745..47712	-	1968	WP_163426525.1	ParB/RepB/Spo0J family partition protein	-
F1745_RS26050 (47776)	47776..48009	-	234	WP_000005985.1	DUF905 family protein	-
F1745_RS26055 (48067)	48067..48606	-	540	WP_163426524.1	single-stranded DNA-binding protein	-
F1745_RS26060 (49079)	49079..49345	-	267	WP_124294726.1	hypothetical protein	-
F1745_RS26065 (49249)	49249..49497	-	249	WP_238014213.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4806.72 Da
• Isoelectric Point: 8.4890

>T139330 WP_096937776.1 NZ_CP043827:c44213-44088 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T139330 NZ_CP058644:5300745-5301059 [Pseudomonas yamanorum]
ATGATATTCATTGAGTCCCCCATCTTCACGGAAGACCTGGGCGAGCTTCTGAGCGATGAAAGTTATCGCGAATTTCAAGA
ATACCTAGCCGAAAACCCAACGGCTGGTGATGTGATCCAAGGCACCAATGGACTGAGGAAAGTCCGCTGGAGCGCTAACG
GGAAAGGTAAGAGTGGTGGGGTTCGGGTGATCTACTTCCACCTTTCAGCGGCTTACCAGATCCGTCTGATCCTGATCTAC
CGTAAGGGGATCAAAGACGATCTTTCCCCAGCGGAAAAGAAAGTACTGGCGGAATTAAACAGAGGGTGGAAATGA

Antitoxin

Nucleotide

• Length: 225 bp

>AT139330 NZ_CP043827:c44514-44290 [Escherichia coli]
TCACACGGATTGCCCGTGAACTGGCTGAACGACCAGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin